Optofluidic Waveguides in Teflon AF-Coated PDMS Microfluidic Channels.
Optofluidic Waveguides in Teflon AF-Coated PDMS Microfluidic Channels.
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DOI：
10.1109/lpt.2009.2022276
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发表时间：
2009-08-01
期刊：
影响因子：
--
通讯作者：
Lo YH
中科院分区：
文献类型：
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作者：
Cho SH;Godin J;Lo YH
本文报道了一种与聚二甲基硅氧烷（PDMS）相容的光流体波导的制备方法。光路遵循微流体通道，这种架构可以最大限度地提高检测效率，并在许多芯片实验室应用中最经济地利用芯片面积。基于PDMS的微流体通道涂覆有具有比水（n = 1.33）低的折射率（n = 1.31）的Teflon无定形含氟聚合物（Teflon AF），以形成水/Teflon AF光波导。在真空泵的驱动下，Teflon AF溶液流过通道，在通道壁上留下薄（5-15 μm）涂层作为光波导的包覆层。该涂覆工艺通过减少Teflon AF包覆层和PDMS器件主体之间的弹性失配来解决旋涂工艺的局限性。我们证明，由此产生的光流体波导限制和引导激光通过液芯通道。此外，当流体流被分离时，这种波导中的光可以被分离。这种新方法使高度集成的生物传感器，如实验室芯片上的流式细胞仪和微制造的荧光激活细胞分选仪与芯片上的激发。
We report a new method for fabricating an optofluidic waveguide that is compatible with polydimethylsiloxane (PDMS). The light path follows the microfluidic channels, an architecture that can maximize detection efficiency and make the most economic use of chip area in many lab-on-chip applications. The PDMS-based microfluidic channels are coated with Teflon amorphous fluoropolymers (Teflon AF) which has a lower refractive index (n = 1.31) than water (n = 1.33) to form a water/Teflon AF optical waveguide. Driven by a vacuum pump, the Teflon AF solution was flowed through the channels, leaving a thin (5–15 µm) layer of coating on the channel wall as the cladding layer of optical waveguides. This coating process resolves the limitations of spin-coating processes by reducing the elasticity mismatch between the Teflon AF cladding layer and the PDMS device body. We demonstrate that the resulting optofluidic waveguide confines and guides the laser light through the liquid core channel. Furthermore, the light in such a waveguide can be split when the fluid flow is split. This new method enables highly integrated biosensors such as lab-on-chip flow cytometers and micro-fabricated fluorescence-activated cell sorter with on-chip excitation.