Laplace field microscopy for label-free imaging of dynamic biological structures
Laplace field microscopy for label-free imaging of dynamic biological structures
复制标题
DOI:
10.1364/ol.36.004704
复制
发表时间:
2011-12-01
期刊:
影响因子:
3.6
通讯作者:
Popescu, Gabriel
中科院分区:
文献类型:
--
作者:
Kim, Taewoo;Popescu, Gabriel
We present Laplace field microscopy as a method for generating intrinsic contrast of transparent specimens. This technique uses a spatial light modulator to perform the Laplacian of the field in the Fourier plane of a microscope image. The resulting image incorporates phase information and thus renders high contrast images from phase objects. We demonstrate the potential of the method by imaging index-matched beads, unlabeled tissue slices, and dynamic live cells. (C) 2011 Optical Society of America