Quantitative Method to Investigate the Balance between Metabolism and Proteome Biomass: Starting from Glycine
Quantitative Method to Investigate the Balance between Metabolism and Proteome Biomass: Starting from Glycine
复制标题
研究代谢与蛋白质组生物量之间平衡的定量方法：从甘氨酸开始
DOI：
10.1002/anie.201609236
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发表时间：
2016-12-12
影响因子：
16.6
通讯作者：
Raftery, Daniel
中科院分区：
文献类型：
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作者：
Gu, Haiwei;Carroll, Patrick A.;Raftery, Daniel
代谢和生物量之间的平衡在生物系统中是非常重要的，然而，迄今为止还没有定量的方法来表征平衡。在本方法学研究中，我们建议使用不同结构域中氨基酸的分布来研究这种平衡。众所周知，生物系统中的内源性或外源性氨基酸被代谢或掺入游离氨基酸（FAAs）或蛋白质组氨基酸（PAAs）。使用甘氨酸（Gly）作为一个例子，我们展示了一种新的方法来准确地确定使用血清，尿液和细胞样品中的各种域中的氨基酸的量。正如所料，血清和尿液中FAA-和PAA-Gly的分布有很大不同。使用Tet 21 N人神经母细胞瘤细胞，我们还发现Myc（癌基因）诱导的代谢重编程包括更高的代谢Gly速率，这提供了额外的证据，即增殖细胞的代谢适应于促进产生新细胞。因此，预计我们的方法将是非常有价值的代谢和生物量平衡的进一步研究，这将导致更好地了解人类癌症。
The balance between metabolism and biomass is very important in biological systems; however, to date there has been no quantitative method to characterize the balance. In this methodological study, we propose to use the distribution of amino acids in different domains to investigate this balance. It is well known that endogenous or exogenous amino acids in a biological system are either metabolized or incorporated into free amino acids (FAAs) or proteome amino acids (PAAs). Using glycine (Gly) as an example, we demonstrate a novel method to accurately determine the amounts of amino acids in various domains using serum, urine, and cell samples. As expected, serum and urine had very different distributions of FAA-and PAA-Gly. Using Tet21N human neuroblastoma cells, we also found that Myc(oncogene)-induced metabolic reprogramming included a higher rate of metabolizing Gly, which provides additional evidence that the metabolism of proliferating cells is adapted to facilitate producing new cells. It is therefore anticipated that our method will be very valuable for further studies of the metabolism and biomass balance that will lead to a better understanding of human cancers.