Comprehensive measurements of hydroxylinoleate and hydroxyarachidonate isomers in blood samples from primary open-angle glaucoma patients and controls
Comprehensive measurements of hydroxylinoleate and hydroxyarachidonate isomers in blood samples from primary open-angle glaucoma patients and controls
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DOI：
10.1038/s41598-018-36952-6
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发表时间：
2019-02-18
影响因子：
4.6
通讯作者：
Yoshida, Yasukazu
中科院分区：
文献类型：
--
作者：
Umeno, Aya;Tanito, Masaki;Yoshida, Yasukazu
我们以前报道过，根据铁还原活性的测量，系统抗氧化能力降低与原发性开角型青光眼(POAG)和剥脱综合征的发病有关。为探讨POAG的发病机制，本研究通过测定血清羟亚油酸(HODE)和羟基花生四烯酸(HETE)同分异构体的含量，探讨POAG的发病机制。POAG患者(n=198)根据眼压分为正常青光眼组和高眼压性青光眼组(n=84和114)。POAG组血清总HODE(/亚油酸)和HETE(/花生四烯酸)水平(分别为211.9+/-143.0和181.0/-164.1 mU/m ol)显著高于对照组(167.1/-105.2和132.5+/-139.7 mU/m o l，p=0.0025和0.0101)。在调整了人口统计学参数的差异后，多变量分析进一步证实了HODES/HETE与青光眼之间的关联。在HODE异构体中，POAG组9-和13-(Z，E)-HoDES(p=0.0014)和单线态氧专一性产物(10-和12-(Z，E)-HoDes，p=0.0345)水平高于对照组，而自由基介导的氧化专一性产物(9-和13-(E，E)-HoDes，p=0.0557)略高于对照组。酶和单线态氧介导的脂肪酸氧化可能是青光眼患者氧化过程的主要途径。
We previously reported that lower systemic antioxidant capacity is involved in primary open-angle glaucoma (POAG) and exfoliation syndrome pathogeneses as measured by ferric-reducing activity. In the present study, we measured hydroxylinoleate (HODE) and hydroxyarachidonate (HETE) isomer serum levels after sample reduction and saponification to investigate POAG pathogenesis. POAG patients (n = 198) were recruited and divided into normal- and high-tension glaucoma groups (n = 84 and 114, respectively) depending on intraocular pressure. Total HODE (/linoleic acid) and HETE (/arachidonic acid) serum levels were significantly higher in the POAG group (211.9 +/- 143.0 and 181.0 +/- 164.1 mu mol/mol, respectively) than in controls (167.1 +/- 105.2 and 132.5 +/- 139.7 mu mol/mol, p = 0.0025 and 0.0101, respectively). The associations between HODEs/HETEs and glaucoma were further confirmed by multivariate analyses after adjusting for differences in demographic parameters. Among the HODE isomers, the levels of 9- and 13-(Z,E)-HODEs (p = 0.0014) and singlet oxygen-specific products (i.e., 10- and 12-(Z,E)-HODEs, p = 0.0345) were higher in the POAG group than in controls, while free radical-mediated oxidation-specific products (i.e., 9- and 13-(E, E)-HODEs, p = 0.0557) demonstrated a marginal difference. Enzymatic and singlet oxygen-mediated fatty acid oxidation may be major pathways of oxidation process in glaucoma subjects.