Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids
Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids
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DOI：
10.1021/acssynbio.7b00265
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发表时间：
2018-01-01
影响因子：
4.7
通讯作者：
Driessen, Arnold J. M.
中科院分区：
文献类型：
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作者：
Exterkate, Marten;Caforio, Antonella;Driessen, Arnold J. M.
将细胞定义为生命实体的关键方面之一是其自我繁殖的能力。在此过程中，膜生物发生是一个重要因素。在这里，我们开发了一种基于八种酶级联的体外磷脂生物合成途径，从简单的脂肪酸构件和 3-磷酸甘油开始。重构的系统产生芳基链和极性头基组成不同的多种磷脂种类。由于高保真度和多功能性，可以同时实现脂肪酸底物完全转化为多种磷脂种类，从而实现膜扩张，这是合成最小细胞的第一步。
One of the key aspects that defines a cell as a living entity is its ability to self-reproduce. In this process, membrane biogenesis is an essential element. Here, we developed an in vitro phospholipid biosynthesis pathway based on a cascade of eight enzymes, starting from simple fatty acid building blocks and glycerol 3-phosphate. The reconstituted system yields multiple phospholipid species that vary in aryl-chain and polar headgroup compositions. Due to the high fidelity and versatility, complete conversion of the fatty acid substrates into multiple phospholipid species is achieved simultaneously, leading to membrane expansion as a first step toward a synthetic minimal cell.