Leukemogenesis by Gross passage A murine leukemia virus: expression of viruses with recombinant env genes in transformed cells.
Leukemogenesis by Gross passage A murine leukemia virus: expression of viruses with recombinant env genes in transformed cells.
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通过总代的白血病发生鼠白血病病毒：在转化细胞中表达具有重组env基因的病毒。
DOI：
10.1073/pnas.79.12.3872
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发表时间：
1982
影响因子：
11.1
通讯作者：
O'Donnell,PV
中科院分区：
文献类型：
--
作者：
Famulari,NG;Koehne,CF;O'Donnell,PV
已证明来自C3 Hf/Bi小鼠白血病提取物的小鼠白血病病毒（MuLV）是一种病毒复合物，由亲嗜性、异嗜性和重组双嗜性MuLV组分组成。这三种病毒组分的生物化学区分的分子量和肽图的差异，其主要的env基因产物在体内和体外感染细胞中合成。在C3 Hf/Bi小鼠中通过总传代A病毒提取物和通过体外分离的单个亲嗜性和重组MuLV组分诱导的原发性白血病中研究病毒表达。我们的研究结果表明，表达的重组MuLV组件的总传代A病毒复合物是必要的，足以诱导白血病在C3 Hf/Bi小鼠。相反，亲嗜性病毒组分诱导白血病似乎涉及产生具有重组双嗜性MuLV特征的第二种病毒。
Gross passage A murine leukemia virus (MuLV) derived from extracts of C3Hf/Bi mouse leukemias has been shown to be a virus complex consisting of ecotropic, xenotropic, and recombinant, dualtropic MuLV components. The three virus components were distinguished biochemically by differences in the molecular weights and peptide maps of their primary env gene products synthesized in infected cells in vivo and in vitro. Virus expression was studied in primary leukemias induced in C3Hf/Bi mice by Gross passage A virus extracts and by the individual ecotropic and recombinant MuLV components that were isolated in vitro. Our findings suggest that expression of the recombinant MuLV component of the Gross passage A virus complex is necessary and sufficient for the induction of leukemias in C3Hf/Bi mice. In contrast, induction of leukemias by the ecotropic virus component appears to involve generation of a second virus with characteristics of recombinant, dualtropic MuLV.