Trace Oxygen Affects Osmium Redox Polymer Synthesis for Wired Enzymatic Biosensors.
Trace Oxygen Affects Osmium Redox Polymer Synthesis for Wired Enzymatic Biosensors.
复制标题
微量氧气影响有线酶生物传感器的锇氧化还原聚合物合成。
DOI：
10.1149/1945-7111/ac42a0
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发表时间：
2022
影响因子：
3.9
通讯作者：
Cliffel,DavidE
中科院分区：
文献类型：
--
作者：
Calhoun,MargaretC;Stachurski,ChristopherD;Winn,SaraL;Gizzie,EvanA;Daniel,AaronW;Schley,NathanD;Cliffel,DavidE
利用酶的电化学传感器是一种灵敏、廉价的检测生物相关分析物的方法。这些传感器根据其结构和信号传输方法进行分类。I型传感器由电极表面上的交联酶组成，并且可能受到副产物和其他生物分析物的干扰。然而，II型传感器通过添加氧化还原聚合物层来帮助缓解这个问题，从而帮助信号转导，从而最大限度地减少干扰。负载锇的聚（乙烯酰咪唑）聚合物（Os-PVI）通常用于成功的结果，当与酶结合时产生II型传感器。我们最初合成这种聚合物的尝试导致了一种意想不到的锇前体，它具有荧光和氧化还原特性，与期望的Os-PVI聚合物不匹配。在Os络合物前驱体合成过程中小心地排除氧是必要的，以避免这种意想不到的含氧Os前驱体，这在以前的质谱研究中已经看到。所有的前体和锇聚合物都用1h NMR，荧光，质谱和循环伏安法进行了表征，以更好地了解这些化合物并协助建立新的传感器。
Electrochemical sensors that utilize enzymes are a sensitive, inexpensive means of detecting biologically relevant analytes. These sensors are categorized based on their construction and method of signal transport. Type I sensors consist of a crosslinked enzyme on an electrode surface and are potentially subject to interference from byproducts and other biological analytes. However, type II sensors help alleviate this problem with the addition of a redox polymer layer that assists in signal transduction, thus minimizing interferences. An osmium-loaded poly (vinylimidazole) polymer (Os-PVI) is commonly used with successful results, and when combined with an enzyme yields a type II sensor. Our initial attempts at the synthesis of this polymer resulted in an unexpected osmium precursor, which had fluorescent and redox properties that did not match with the desired Os-PVI polymer. Careful exclusion of oxygen during the Os complex precursor synthesis was necessary to avoid this unexpected oxygen containing Os-precursor, which had been seen previously in mass spectrometry studies. All precursors and osmium polymers were characterized with 1 H NMR, fluorescence, mass spectrometry, and cyclic voltammetry to provide a better understanding of these compounds and assist in the building of new sensors.