Distribution of OL-protocadherin protein in correlation with specific neural compartments and local circuits in the postnatal mouse brain
Distribution of OL-protocadherin protein in correlation with specific neural compartments and local circuits in the postnatal mouse brain
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DOI：
10.1016/s0306-4522(02)00944-2
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Hirano, S
中科院分区：
文献类型：
--
作者：
Aoki, E;Kimura, R;Hirano, S
OL-原钙粘蛋白 (OL-pc) 是一种属于钙粘蛋白超家族的细胞粘附分子。先前的一项研究表明，OL-pc mRNA 的表达对某些脑核（包括嗅觉和边缘系统）具有特异性，因此表明它参与神经回路的形成（Hirano 等，1999）。在这里，我们通过免疫组织化学检查了 OL-pc 蛋白在出生后小鼠大脑中的分布，以证实这种作用的可能性。结果表明，该蛋白质可以映射到许多脑区，包括脑核和更高的细分，正如之前观察到的 mRNA 表达模式一样。分布的尖锐边界经常出现在脚间核、小脑皮质和下橄榄等区域。此外，在一些纤维中检测到了先前使用原位杂交的研究无法检测到的蛋白质。例如，在髓纹、终纹、反屈束、视束和下丘脑辐射中发现了显着的染色，这些结构似乎连接着 OL-pc 阳性脑区。这些OL-pc阳性脑核和纤维束与功能系统的一些局部回路一致，例如嗅觉系统、黑质纹状体投射、橄榄小脑投射和视觉系统。这些结果支持了 OL-pc 参与发育中大脑中特定神经区室和回路的形成的可能性。 (C) 2003 国际广播组织。由爱思唯尔科学有限公司出版。保留所有权利。
OL-protocadherin (OL-pc) is a cell adhesion molecule that belongs to the cadherin superfamily. A previous study showed that expression of OL-pc mRNA was specific to certain brain nuclei including those of the olfactory and limbic systems, thus suggesting its involvement in neural circuit formation (Hirano et al., 1999). Here, we examined the distribution of OL-pc protein in the postnatal mouse brain by immunohistochemistry to confirm the possibility of such a role. The results showed that the protein could be mapped to many brain compartments including brain nuclei and higher subdivisions as previously observed for the expression pattern of the mRNA. Sharp boundaries of the distribution were often seen in areas such as the interpedunclar nucleus, cerebellar cortex, and inferior olive. In addition, the protein was detected in some fibers that could not be examined by the previous study using in situ hybridization. For example, prominent staining was noted in the stria medularis, stria terminalis, fasciculus retroflexus, optic tract, and inferior thalamic radiation, structures that seem to connect OL-pc-positive brain regions. These OL-pc-positive brain nuclei and fiber tracts coincide with some local circuits of functional systems such as the olfactory system, nigrostriatal projection, olivo-cerebellar projection, and visual system. These results support the possibility that OL-pc is involved in the formation of specific neural compartments and circuits in the developing brain. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.