Frequent detection of minimal residual disease by use of the polymerase chain reaction in long-term survivors after bone marrow transplantation for chronic myeloid leukemia.
Frequent detection of minimal residual disease by use of the polymerase chain reaction in long-term survivors after bone marrow transplantation for chronic myeloid leukemia.
复制标题
使用聚合酶链反应在慢性粒细胞白血病骨髓移植后的长期幸存者中频繁检测微小残留病。
作者：
J. Pignon;T. Henni;S. Amselem;M. Vidaud;P. Duquesnoy;J. Vernant;M. Kuentz;C. Cordonnier;H. Rochant;M. Goossens
聚合酶链式反应(PCR)允许检测最少量的核序列，并已成功地用于检测慢性粒细胞白血病特异的bcr/abl转录本。我们研究了17名接受异基因骨髓移植治疗CML的患者的血液样本，采用一种基于改进的聚合酶链式反应的分析方法来检测白血病的mRNA。这种套式PCR技术被发现是高度敏感的，在17名患者中的16名患者中检测到嵌合的bcr/abl转录本，其中包括几名长期幸存者。细胞遗传学技术未能检测到Ph有丝分裂。骨髓移植后长期持续的bcr/abl转录本的临床意义尚不清楚，还有待进一步的研究来阐明。
The polymerase chain reaction (PCR) allows the detection of minimal amounts of nucleic sequences and has been successfully used to test for the chronic myeloid leukemia-specific bcr/abl transcripts. We studied blood samples from 17 patients who had undergone allogeneic bone marrow transplantation for CML, using a modified polymerase chain reaction-based assay for the detection of leukemic mRNA. This nested PCR technique was found to be highly sensitive, detecting the chimeric bcr/abl transcript in 16 of 17 patients including several long-term survivors. Cytogenetic techniques failed to detect Ph mitoses. The clinical significance of the persisting bcr/abl transcript for long periods following BMT is poorly understood and remains to be elucidated by further studies.