Live cell labeling of glial progenitor cells using targeted quantum dots.
Live cell labeling of glial progenitor cells using targeted quantum dots.
复制标题
使用靶向量子点对神经胶质祖细胞进行活细胞标记。
DOI：
10.1007/s10439-009-9703-4
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发表时间：
2009
影响因子：
3.8
通讯作者：
Vazquez,Maribel
中科院分区：
文献类型：
--
作者：
Sabharwal,Nidhi;Holland,EricC;Vazquez,Maribel
本研究描述了靶向量子点（T-QDs）作为标记过表达血小板衍生生长因子（PDGF）及其受体PDGFR （GPCPDGF）的胶质祖细胞（GPCs）的生物标志物的发展。PDGFR在胶质瘤的发育和生长中起着至关重要的作用，并且还影响多种生物学过程，如细胞迁移和胚胎发育。T-QDs是用链霉亲和素偶联量子点（S-QDs）与生物素化抗体构建的，并通过阳离子脂质体脂质体转染，用于标记活的培养gpcpdgf细胞的细胞内和细胞外结构域。共聚焦研究表明，在实时信号事件中，活细胞内成功的细胞内和细胞外靶向标记似乎不会影响上游PDGFR动态。此外，T-QDs对gpcpdgf细胞无毒，并且在6天内不改变细胞活力或增殖。这些结果提出了T-QDs作为活细胞内蛋白质群成像和跟踪的超敏感试剂的新应用，这将使未来实时研究致癌信号事件的机制成为可能。
This study describes the development of targeted quantum dots (T-QDs) as biomarkers for the labeling of glial progenitor cells (GPCs) that over express platelet derived growth factor (PDGF) and its receptor PDGFR (GPCPDGF). PDGFR plays a critical role in glioma development and growth, and is also known to affect multiple biological processes such as cell migration and embryonic development. T-QDs were developed using streptavidin-conjugated quantum dots (S-QDs) with biotinylated antibodies and utilized to label the intracellular and extracellular domains of live, cultured GPCPDGFcells via lipofection with cationic liposomes. Confocal studies illustrate successful intracellular and extracellular targeted labeling within live cells that does not appear to impact upstream PDGFR dynamics during real-time signaling events. Further, T-QDs were nontoxic to GPCPDGFcells, and did not alter cell viability or proliferation over the course of 6 days. These results raise new applications for T-QDs as ultra sensitive agents for imaging and tracking of protein populations within live cells, which that will enable future mechanistic study of oncogenic signaling events in real-time.