Transcriptional and Functional Classification of the GOLVEN/ROOT GROWTH FACTOR/CLE-Like Signaling Peptides Reveals Their Role in Lateral Root and Hair Formation
Transcriptional and Functional Classification of the GOLVEN/ROOT GROWTH FACTOR/CLE-Like Signaling Peptides Reveals Their Role in Lateral Root and Hair Formation
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DOI：
10.1104/pp.112.206029
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发表时间：
2013-02-01
期刊：
影响因子：
7.4
通讯作者：
Hilson, Pierre
中科院分区：
文献类型：
--
作者：
Fernandez, Ana;Drozdzecki, Andrzej;Hilson, Pierre
GOLVEN（GLV）/根生长因子/CLE样小信号肽家族由拟南芥（Arabidopsis thaliana）中的11个基因编码。它们中的一些已经被证明控制根分生组织的维持，生长素通量和向重力反应。作为详细分析其功能的基础，我们确定了11个GLV基因的表达结构域与启动子报告线。尽管GLV基因在所有被检测的植物部位都有活性，但它们具有高度特异性的转录模式，通常仅限于根和茎以及营养和生殖组织中的极少数细胞或细胞类型。通过比较分析功能获得和丧失突变体诱导的根表型或用GLV衍生的合成肽处理，进一步研究了GLV的功能。我们确定了与主根中基因表达结构域相关的功能类别，并表明不同的GLV信号触发不同的下游途径。有趣的是，在侧根发育的早期阶段转录的GLV基因在过表达时强烈抑制根分枝。此外，转录模式与突变体表型一起指出GLV 4和GLV 8参与根毛形成。总体而言，我们的数据表明，9个GLV基因根据其在根中的表达和功能形成三个亚组，并提供了一个全面的框架来研究GLV信号肽在植物发育中的作用。
The GOLVEN (GLV)/ROOT GROWTH FACTORS/CLE-Like small signaling peptide family is encoded by 11 genes in Arabidopsis (Arabidopsis thaliana). Some of them have already been shown to control root meristem maintenance, auxin fluxes, and gravitropic responses. As a basis for the detailed analysis of their function, we determined the expression domains for each of the 11 GLV genes with promoter-reporter lines. Although they are collectively active in all examined plant parts, GLV genes have highly specific transcription patterns, generally restricted to very few cells or cell types in the root and shoot and in vegetative and reproductive tissues. GLV functions were further investigated with the comparative analysis of root phenotypes induced by gain-and loss-of-function mutants or in treatments with GLV-derived synthetic peptides. We identified functional classes that relate to the gene expression domains in the primary root and suggest that different GLV signals trigger distinct downstream pathways. Interestingly, GLV genes transcribed at the early stages of lateral root development strongly inhibited root branching when overexpressed. Furthermore, transcription patterns together with mutant phenotypes pointed to the involvement of GLV4 and GLV8 in root hair formation. Overall, our data suggest that nine GLV genes form three subgroups according to their expression and function within the root and offer a comprehensive framework to study the role of the GLV signaling peptides in plant development.