Isolation and characterization of the bovine hypothalamic growth hormone releasing factor.
Isolation and characterization of the bovine hypothalamic growth hormone releasing factor.
复制标题
牛下丘脑生长激素释放因子的分离和表征。
DOI：
10.1016/0006-291x(83)91664-9
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发表时间：
1983
影响因子：
3.1
通讯作者：
Guillemin,R
中科院分区：
文献类型：
--
作者：
Esch,F;Böhlen,P;Ling,N;Brazeau,P;Guillemin,R
采用酸提取、免疫亲和层析、凝胶过滤和两步反相高效液相色谱法，从500头牛下丘脑中分离到一个由44个氨基酸组成的内源性生长激素释放肽。生长激素释放因子的结构特征通过气相序列分析。天然肽和合成复制品的反相液相色谱显示，该分子在其羧基末端具有酰胺而不是游离酸。肽的结构确定为：Tyr Ala-Asp-Ala-Ile- Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Lys-Val-Arg-Leu-NH 2，使用约2 nmol材料。
A 44 amino acid peptide with high intrinsic growth hormone releasing activity was isolated from 500 bovine hypothalami by means of acid extraction, immunoaffinity chromatography, gel filtration, and two steps of reverse phase HPLC. The growth hormone releasing factor was structurally characterized by gas phase sequence analysis. Reverse phase liquid chromatography of the native peptide and synthetic replicates showed that the molecule possesses an amide rather than a free acid at its carboxyl terminus. The structure of the peptide was established as: Tyr Ala-Asp-Ala-Ile- Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln- Asp-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Lys-Val-Arg- Leu-NH2using approximately 2 nmol of material.