Beneficial effects of ventromedial hypothalamus (VMH) lesioning on functionand morphology of the liver after hepatectomy in rats
Beneficial effects of ventromedial hypothalamus (VMH) lesioning on functionand morphology of the liver after hepatectomy in rats
复制标题
下丘脑腹内侧（VMH）损伤对大鼠肝切除术后肝脏功能和形态的有益影响
DOI：
10.1016/j.brainres.2011.09.012
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发表时间：
2011
期刊：
影响因子：
2.9
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Lee;EY.;Inoue;S.;Senoo;A.;Shimizu;H.;Suzuki Y.;Ishizuka;N.;Imazeki;N.;Sasaki;K.;Kako;M.;Osaka;T.;Miki;T.
肝脏具有高再生能力，并且在部分肝切除术后不久通过增加肝细胞的增殖和代谢修饰恢复其质量和功能。肝细胞的增殖可以由肝切除后其质量减少或由神经因素包括下丘脑腹内侧（VMH）损伤触发。在本研究中，我们研究了VMH毁损对肝切除大鼠肝再生的影响，通过评估肝功能和形态学。我们发现部分肝切除引起的功能缺陷[凝血酶原时间（PT）延长，吲哚菁绿色（ICG）保留增加，PAS（过碘酸-希夫染色）阳性肝细胞减少]在术后1周通过VMH损伤得到恢复，而这些改变在术后4周消失。在形态学上，脂质微滴被认为是通过为细胞增殖提供燃料来维持连续肝功能的重要物质，在部分肝切除后早期（1d）发现其在肝细胞中积聚。有趣的是，在VMH损伤的大鼠中也检测到这种脂质微滴，并且在VMH损伤的肝切除大鼠中更丰富，直到手术后1周。总之，我们的研究结果表明，VMH损伤大鼠肝部分切除术后，通过促进细胞增殖过程促进肝脏解剖和功能的恢复。
Liver has a high regenerative capacity and restores its mass and function shortly after partial hepatectomy through increased proliferation and metabolic modification of hepatocytes. The proliferation of hepatocytes can be triggered by its mass reduction after hepatectomy or by the neural factors including lesioning of the ventromedial hypothalamus (VMH). In the present study, we examined the effect of VMH lesioning on liver regeneration in hepatectomized rats by evaluating liver function and morphology. We found that functional deficits caused by partial hepatectomy [prolonged prothrombin time (PT), increased indocyanine green (ICG) retention, and decrease in PAS (periodic Acid-Schiff staining)-positive hepatocytes] were restored by VMH lesioning at 1week after the surgery, whereas these alterations disappeared at 4weeks. Morphologically, lipid microdroplets, which are considered to be important for maintaining contiguous liver function via supplying fuel for cell proliferation, were found to accumulate in hepatocytes of the hepatectomized rats at early period (1day) after partial hepatectomy. Interestingly, such lipid microdroplets were also detected in the VMH lesioned rats and the more abundantly in the VMH lesioned, hepatectomized rats up to 1week after the surgery. In conclusion, our results suggest that VMH lesioning in rats promotes recovery of liver anatomically and functionally after partial hepatectomy by promoting cell proliferation process.