Effects of 7-methylxanthine on the sclera in form deprivation myopia in guinea pigs
Effects of 7-methylxanthine on the sclera in form deprivation myopia in guinea pigs
复制标题
DOI：
10.1111/j.1755-3768.2009.01688.x
复制
发表时间：
2011-06-01
影响因子：
3.4
通讯作者：
Ge, Jian
中科院分区：
文献类型：
--
作者：
Cui, Dongmei;Trier, Klaus;Ge, Jian
目的：本研究旨在探讨腺苷受体拮抗剂7-甲基黄嘌呤（7-MX）对3周龄豚鼠形觉剥夺性近视的影响。（300 mg/kg体重; n = 7）或媒介物对照（与7-MX等体积的盐水; n = 7）。对照组（n = 6）不进行形觉剥夺。在开始和21天后测量眼屈光度、眼轴长度和体重。后巩膜的厚度通过光学显微镜和胶原纤维直径的内层，中层和外层的巩膜，通过电子显微镜测量。结果：在车辆对照组，21天的MD产生显着量的近视，轴向伸长，后巩膜变薄和变薄的中位胶原纤维直径在后巩膜相对于对侧眼睛。然而，在用7-MX喂养的豚鼠中，与溶剂对照动物相比，形觉剥夺产生的近视和轴向伸长显著减少。7-MX给药动物的MD眼和对侧眼均显示后巩膜增厚。在7-MX处理的动物中，后巩膜中的胶原纤维直径中值未因形觉剥夺而减小。用7-MX治疗似乎不仅使豚鼠的近视量减少约50%并消除由形觉剥夺诱导的眼睛伸长，而且还防止形觉剥夺近视相关的巩膜变化，例如巩膜变薄和后巩膜中胶原纤维直径变薄。
Purpose: The aim of this study was to determine the effect of the adenosine receptor antagonist 7-methylxanthine (7-MX) on form deprivation myopia in 3-week-old guinea pigs.Methods: Two groups of 3-week-old guinea pigs were subjected to monocular deprivation (MD) using a diffuser and fed either 7-MX (300 mg/kg body weight; n = 7) or vehicle control (saline at an equal volume to 7-MX; n = 7). A control group (n = 6) was not subjected to form deprivation. Ocular refraction, axial length and body weight were measured at the start and after 21 days. The thickness of the posterior sclera was measured by light microscopy and the collagen fibril diameter in the inner, middle and outer layers of the sclera was measured by electron microscopy.Results: In the vehicle control group, 21 days of MD produced significant amounts of myopia, axial elongation, thinning of the posterior sclera and thinning of the median collagen fibril diameter in the posterior sclera relative to the contralateral eyes. In the guinea pigs fed with 7-MX, however, form deprivation produced significantly less myopia and axial elongation compared with vehicle control animals. The 7-MX-treated animals exhibited a thickening of the posterior sclera in both the MD eye and the contralateral eye. In the 7-MX-treated animals, the median collagen fibril diameter in the posterior sclera was not reduced by form deprivation.Conclusions: Treatment with 7-MX appears to not only decrease the amount of myopia by around 50% and eliminate the eye elongation induced by form deprivation in guinea pigs, but also to prevent form deprivation myopia-related scleral changes, such as thinning of the sclera and thinning of the collagen fibril diameter in the posterior sclera.