Study of interactions between DNA-ethidium bromide (EB) and DNA-acridine orange (AO), in solution, using hanging mercury drop electrode (HMDE)
Study of interactions between DNA-ethidium bromide (EB) and DNA-acridine orange (AO), in solution, using hanging mercury drop electrode (HMDE)
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DOI：
10.1016/s0039-9140(03)00238-8
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发表时间：
2003-10-17
期刊：
影响因子：
6.1
通讯作者：
Tzimou-Tsitouridou, R
中科院分区：
文献类型：
--
作者：
Gherghi, IC;Girousi, ST;Tzimou-Tsitouridou, R
在悬汞电极上，用交流伏安法研究了溴化乙锭（EB）和吖啶橙子（AO）与双链（ds）、热变性（ss）和超螺旋（sc）DNA在溶液中的相互作用.它们与DNA的相互作用显示出时间依赖性和完全不同。dsDNA形式的峰2（峰在-1.20 V）处的变化和scDNA形式的峰3（峰在-1.42 V）的出现被呈现为宣布EB和AO与DNA相互作用的不同机制的标准。此外，由于DNA和AO相互作用，在约-0.44 V处出现新峰，区分了所研究的行为。这些化合物的电化学行为的比较突出了相互作用机制的差异。(C)2003 Elsevier Science B. V.保留所有权利。
The interaction of ethidium bromide (EB) and acridine orange (AO) with double stranded (ds), thermally denatured (ss) and supercoiled (sc) DNA, in solution, was studied by alternating current voltammetry (AC voltammetry) at the hanging mercury drop electrode (HMDE) in 0.3 M NaCl+50 mM sodium phosphate buffer (pH 8.5). Their interaction with DNA is shown to be time dependent and completely different. The changes at peak 2 (peak at - 1.20 V) of dsDNA form and the appearance of peak 3 (peak at - 1.42 V) in scDNA form are presented as criteria declaring the different mechanism of interaction of EB and AO with DNA. Additionally, the appearance of a new peak at around -0.44 V as a result of DNA and AO interaction, differentiates the studied behaviors. The comparison of the electrochemical behaviors of these compounds highlights the differences in the mechanism of interaction. (C) 2003 Elsevier Science B.V. All rights reserved.