Daily coordination of orexinergic gating in the rat superior colliculus-Implications for intrinsic clock activities in the visual system
Daily coordination of orexinergic gating in the rat superior colliculus-Implications for intrinsic clock activities in the visual system
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DOI：
10.1096/fj.202100779rr
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发表时间：
2021-10-01
期刊：
影响因子：
4.8
通讯作者：
Lewandowski, Marian Henryk
中科院分区：
文献类型：
--
作者：
Chrobok, Lukasz;Jeczmien-Lazur, Jagoda Stanislawa;Lewandowski, Marian Henryk
食欲素能系统为多个大脑中心提供兴奋以促进行为唤醒，其功能障碍导致发作性睡病，嗜睡，特别是幻视。由于生物钟是日常唤醒的基础，因此期望食欲素系统与其目标皮层下视觉系统（包括上级丘（SC））之间的定时协调。在这里，我们使用的组合电生理，免疫组织化学，和分子的方法在24小时内，连同神经束追踪的方法来研究食欲素系统和啮齿动物SC之间的日常协调。更高的orexinergic输入被发现在SC的浅层，在夜间沉默的自发放电在这个视觉脑区的时间。我们确定自主的日常和昼夜节律的时钟基因在SC中的表达，这可能是这些昼夜变化的基础。此外，我们建立了下丘脑外侧起源的食欲素神经支配的SC和SC神经元强烈响应食欲素A通过OX2受体兴奋和GABA（A）受体依赖性抑制方式。总之，我们的证据阐明了内在和外在的时钟机制的组合，形状的视觉层的SC的日常功能。
The orexinergic system delivers excitation for multiple brain centers to facilitate behavioral arousal, with its malfunction resulting in narcolepsy, somnolence, and notably, visual hallucinations. Since the circadian clock underlies the daily arousal, a timed coordination is expected between the orexin system and its target subcortical visual system, including the superior colliculus (SC). Here, we use a combination of electrophysiological, immunohistochemical, and molecular approaches across 24 h, together with the neuronal tract-tracing methods to investigate the daily coordination between the orexin system and the rodent SC. Higher orexinergic input was found to occur nocturnally in the superficial layers of the SC, in time for nocturnal silencing of spontaneous firing in this visual brain area. We identify autonomous daily and circadian expression of clock genes in the SC, which may underlie these day-night changes. Additionally, we establish the lateral hypothalamic origin of the orexin innervation to the SC and that the SC neurons robustly respond to orexin A via OX2 receptor in both excitatory and GABA(A) receptor-dependent inhibitory manners. Together, our evidence elucidates the combination of intrinsic and extrinsic clock mechanisms that shape the daily function of the visual layers of the SC.