Neural differentiation regulated by biomimetic surfaces presenting motifs of extracellular matrix proteins
Neural differentiation regulated by biomimetic surfaces presenting motifs of extracellular matrix proteins
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DOI：
10.1002/jbm.a.32585
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发表时间：
2010-06-01
影响因子：
4.9
通讯作者：
Przyborski, S. A.
中科院分区：
文献类型：
--
作者：
Cooke, M. J.;Zahir, T.;Przyborski, S. A.
细胞与细胞外基质(ECM)之间的相互作用是发育过程中必不可少的。为了阐明ECM蛋白在细胞分化中的作用，我们开发了以受控方式展示特定ECM多肽基序的仿生表面。胶原、纤维连接蛋白和层粘连蛋白的ECM结构域通过分化PC12细胞影响神经突起的形成。这些多肽序列对成体神经干/祖细胞发育的影响也被测试。在这个系统中，I型胶原和纤维连接蛋白诱导形成β-Ill-微管蛋白阳性细胞，而IV型胶原抑制这种分化。由多种多肽类型组成的仿生表面使得研究各种ECM基序的组合效应成为可能。由于分离研究的ECM多肽的协同作用，显示结合基序的表面通常是可预测的。例如，纤维连接蛋白和层粘连蛋白的相加作用导致β-Ill-微管蛋白阳性细胞的表达增加，而IV型胶原结构域的负面作用被I型胶原的共同表达所抵消。然而，某些ECM结构域的同时表达是不可预测的。这些数据突显了细胞对组合的ECM信号反应的复杂性，以及单独和联合研究ECM结构域功能的必要性。(C)2009年Wiley期刊，Inc.《生物材料》93A：824-832,2010
The interaction between cells and the extracellular matrix (ECM) is essential during development. To elucidate the function of ECM proteins on cell differentiation, we developed biomimetic surfaces that display specific ECM peptide motifs in a controlled manner. Presentation of ECM domains for collagen, fibronectin, and laminin influenced the formation of neurites by differentiating PC12 cells. The effect of these peptide sequences was also tested on the development of adult neural stem/progenitor cells. In this system, collagen I and fibronectin induced the formation of beta-Ill-tubulin positive cells, whereas collagen IV reduced such differentiation. Biomimetic surfaces composed of multiple peptide types enabled the combinatorial effects of various ECM motifs to be studied. Surfaces displaying combined motifs were often predictable as a result of the synergistic effects of ECM peptides studied in isolation. For example, the additive effects of fibronectin and laminin resulted in greater expression of beta-Ill-tubulin positive cells, whereas the negative effect of the collagen IV domain was canceled out by coexpression of collagen I. However, simultaneous expression of certain ECM domains was less predictable. These data highlight the complexity of the cellular response to combined ECM signals and the need to study the function of ECM domains individually and in combination. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 824-832, 2010