Reestablishment of damaged adult motor pathways by grafted embryonic cortical neurons
Reestablishment of damaged adult motor pathways by grafted embryonic cortical neurons
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DOI：
10.1038/nn1970
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发表时间：
2007-10-01
影响因子：
25
通讯作者：
Jaber, Mohamed
中科院分区：
文献类型：
--
作者：
Gaillard, Afsaneh;Prestoz, Laetitia;Jaber, Mohamed
成年运动皮层的损伤导致运动功能的严重和经常不可逆的缺陷。胚胎皮层神经元移植到受损的成年运动皮层以前被证明会诱导部分恢复，但移植传出的报告有所不同，从没有传出的预测稀疏的神经支配。在这里，我们将过度表达绿色荧光蛋白的转基因小鼠的胚胎皮层组织移植到成年小鼠受损的运动皮层中。移植的神经元产生了向适当的皮质和皮质下宿主靶点（包括丘脑和脊髓）的传出投射。这些投射不是移植物和宿主神经元之间细胞融合的结果。宿主和移植神经元形成突触接触，许多移植传出神经有髓鞘。这些发现表明，有大量的解剖重建后，胚胎皮层移植到成人大脑皮层电路。他们认为，神经细胞移植在促进脑损伤后重建方面具有不可预测的潜力。
Damage to the adult motor cortex leads to severe and frequently irreversible deficits in motor function. Transplantation of embryonic cortical neurons into the damaged adult motor cortex was previously shown to induce partial recovery, but reports on graft efferents have varied from no efferent projections to sparse innervation. Here, we grafted embryonic cortical tissue from transgenic mice overexpressing a green fluorescent protein into the damaged motor cortex of adult mice. Grafted neurons developed efferent projections to appropriate cortical and subcortical host targets, including the thalamus and spinal cord. These projections were not a result of cell fusion between the transplant and the host neurons. Host and transplanted neurons formed synaptic contacts and numerous graft efferents were myelinated. These findings demonstrate that there is substantial anatomical reestablishment of cortical circuitry following embryonic cortex grafting into the adult brain. They suggest that there is an unsuspected potential for neural cell transplantation to promote reconstruction after brain injury.