Validation of a method to quantify chromium, cadmium, manganese, nickel and lead in human whole blood, urine, saliva and hair samples by electrothermal atomic absorption spectrometry
Validation of a method to quantify chromium, cadmium, manganese, nickel and lead in human whole blood, urine, saliva and hair samples by electrothermal atomic absorption spectrometry
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DOI：
10.1016/j.aca.2009.11.056
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发表时间：
2010-02-05
影响因子：
6.2
通讯作者：
Gil, F.
中科院分区：
文献类型：
--
作者：
Olmedo, P.;Pla, A.;Gil, F.
对于职业毒理学中重金属暴露的生物监测，通常全血和尿液样品是最广泛使用和接受的基质，以评估内异生物质暴露。头发样品和唾液也感兴趣的职业和环境健康调查，但程序的唾液和头发中的金属测定是非常稀缺的，据我们所知，有没有验证的方法来量化铬，镉，锰，镍和铅在四个不同的人类生物材料（全血，尿液，唾液和腋毛）的原子吸收光谱法（ETAAS）。在本研究中，用于测定全血，尿液，唾液和腋毛中的铬，镉，锰，镍和铅的定量方法进行了验证，根据欧盟通用标准。测定了热解温度和原子化温度。评价的主要参数包括：检测限和定量限、线性范围、重复性、重现性、回收率和不确定度。方法的准确度进行了测试与全血，尿液和头发认证的参考材料和加标样品的回收率是可接受的范围从96.3%到107.8%。(C)2009爱思唯尔有限公司版权所有。
For biological monitoring of heavy metal exposure in occupational toxicology, usually whole blood and urine samples are the most widely used and accepted matrix to assess internal xenobiotic exposure. Hair samples and saliva are also of interest in occupational and environmental health surveys but procedures for the determination of metals in saliva and hair are very scarce and to our knowledge there is no validation of a method to quantify Cr, Cd, Mn, Ni and Pb in four different human biological materials (whole blood, urine, saliva and axilary hair) by electrothermal atomization atomic absorption spectrometry (ETAAS). In the present study, quantification methods for the determination of Cr, Cd, Mn, Ni and Pb in whole blood, urine, saliva and axilary hair were validated according to the EU common standards. Pyrolisis and atomization temperatures have been determined. The main parameters evaluated were: detection and quantification limits, linearity range, repeatability, reproducibility, recovery and uncertainty. Accuracy of the methods was tested with the whole blood, urine and hair certified reference materials and recoveries of the spiked samples were acceptable ranged from 96.3 to 107.8%. (C) 2009 Elsevier B.V. All rights reserved.