The role of serum levels of microRNA-21 and matrix metalloproteinase-9 in patients with acute coronary syndrome
The role of serum levels of microRNA-21 and matrix metalloproteinase-9 in patients with acute coronary syndrome
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DOI：
10.1007/s11010-016-2805-z
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发表时间：
2016-11-01
影响因子：
4.3
通讯作者：
Sarrafzadegan, Nizal
中科院分区：
文献类型：
--
作者：
Darabi, Faramarz;Aghaei, Mahmoud;Sarrafzadegan, Nizal
急性冠脉综合征（ACS）是心血管死亡的主要原因之一。 microRNA-21和基质金属蛋白酶9似乎与心血管疾病的发病机制有关。本研究的目的是探讨循环 miR-21 和 MMP-9 作为 ACS 生物标志物的作用。根据冠状动脉造影和心电图结果，50 名 ACS 患者和 50 名稳定型冠状动脉疾病（稳定 CAD）患者纳入本研究。从患者身上收集样品并保存在-80°C。通过定量实时PCR方法测量血清miR-21基因表达。采用酶联免疫吸附试剂盒测定血清总MMP-9。另外，通过明胶酶谱法测量MMP-9的活性。与稳定的 CAD 患者相比，ACS 患者的 miR-21 水平显着升高（分别 = 0.88 +/- 0.06 和 0.31 +/- 0.08，P < 0.001）。同时，与稳定性CAD患者相比，ACS患者血清MMP-9水平和活性显着升高（324.01+/-17.57和204.6+/-12.39ng/mL，P<0.001，以及2524.5+/-131.3和1280.8+/-19.6单位，P<0.001，分别）。 miR-21表达水平与MMP-9、hs-CRP和年龄呈正相关，与HDL-胆固醇呈负相关（分别为r = 0.33，P < 0.001，r = 0.22，P < 0.031，r = 0.26，P < 0.008，r = -0.32，P < 0.001）。我们得出的结论是，miR-21 血清表达增加和 MMP-9 血清活性升高可能是 ACS 的有用指标。然而，我们建议进行进一步的研究。
Acute coronary syndrome (ACS) is one of the leading causes of cardiovascular death. It seems that microRNA-21 and matrix metalloproteinase-9 implicated in the pathogenesis of cardiovascular diseases. The aim of this study was to investigate the role of circulating miR-21 and MMP-9 as biomarkers for ACS. Based on coronary angiography and electrocardiography results, 50 patients with ACS and 50 patients with stable coronary artery disease (stable CAD) were enrolled in this study. Samples were collected from patients and stored at -80 A degrees C. Serum miR-21 gene expression was measured by quantitative real-time PCR method. Serum total MMP-9 was measured by enzyme-linked immunosorbent assay kit. Also, the activity of MMP-9 was measured by gelatin zymography. Patients with ACS had a significantly higher miR-21 level compared to the stable CAD ( = 0.88 +/- 0.06 and 0.31 +/- 0.08 respectively, P < 0.001). At the same time, the serum levels and activity of MMP-9 were significantly higher in ACS patients compared to those with stable CAD (324.01 +/- 17.57 and 204.6 +/- 12.39 ng/mL, P < 0.001, and 2524.5 +/- 131.3 and 1280.8 +/- 19.6 units, P < 0.001, respectively). miR-21 expression levels were correlated positively with MMP-9, hs-CRP, and age and negatively with HDL-cholesterol (r = 0.33, P < 0.001, r = 0.22, P < 0.031, r = 0.26, P < 0.008, r = -0.32, P < 0.001, respectively). We concluded that increased serum expression of miR-21 and higher serum activity of MMP-9 may be useful indicators for ACS. However, we suggest further studies to be performed.