QTL analysis of sex pheromone blend differences between two closely related moths: Insights into divergence in biosynthetic pathways
QTL analysis of sex pheromone blend differences between two closely related moths: Insights into divergence in biosynthetic pathways
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DOI：
10.1016/j.ibmb.2009.05.002
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发表时间：
2009-08-01
影响因子：
3.8
通讯作者：
Gould, Fred
中科院分区：
文献类型：
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作者：
Groot, Astrid T.;Estock, Marie L.;Gould, Fred
为了了解飞蛾早熟信号的进化，了解这些信号的遗传基础是很重要的。本研究通过对Heliothis virescens （Hv）和Heliothis subflexa （Hs）两种夜蛾进行杂交，并将F-1雌虫与两种亲本进行回交，进行了数量性状位点（QTL）分析。其中一个回交（F-1 x Hs）是我们之前研究的生物重复（Sheck等人，2006），用于检验我们之前研究结果的稳健性。设计Hv回交以揭示Hv性状状态的隐性遗传QTL。本研究证实了先前发现的QTL，同时也报道了新的QTL。最重要的是，我们发现了影响Hs关键性信息素成分Z9-16:Ald的相对较大的QTL。对于Hv的关键性信息素成分Z9-14:Ald，以及次要信息素化合物14:Ald，我们发现了信息素比例变化与预期相反的QTL。将QTL与Hv和Hs信息素化合物的生物合成途径联系起来，暗示了这些预交配信号分化的几个候选基因，其中最重要的是乙酰转移酶、一种或多种去饱和酶、脂肪酰还原酶或酒精氧化酶。2009爱思唯尔有限公司版权所有。
To understand the evolution of premating signals in moths, it is important to know the genetic basis of these signals. We conducted Quantitative Trait Locus (QTL) analysis by hybridizing two noctuid moth species, Heliothis virescens (Hv) and Heliothis subflexa (Hs), and backcrossing the F-1 females to males of both parental species. One of these backcrosses (F-1 x Hs) was a biological replicate of our previous study (Sheck et al, 2006) and served to test the robustness of our previous findings. The backcross to Hv was designed to reveal QTL with recessive inheritance of the Hv character state. This study confirms previously discovered QTL, but also reports new QTL Most importantly, we found relatively large QTL affecting Z9-16:Ald, the critical sex pheromone component of Hs. For Z9-14:Ald, the critical sex pheromone component of Hv, as well as for the minor pheromone compound 14:Ald, we found QTL in which the change in pheromone ratio was opposite-to-expected. linking QTL to the biosynthetic pathways of the pheromone compounds of Hv and Hs implicates several candidate genes in the divergence of these premating signals, the most important of which are acetyl transferase, one or more desaturase(s), and a fatty acyl reductase or alcohol oxidase. (C) 2009 Elsevier Ltd. All rights reserved.