A novel cryptic plasmid pBMB175 from Bacillus thuringiensis subsp tenebrionis YBT-1765
A novel cryptic plasmid pBMB175 from Bacillus thuringiensis subsp tenebrionis YBT-1765
复制标题
DOI：
10.1007/s00203-007-0222-0
复制
发表时间：
2007-07-01
影响因子：
2.8
通讯作者：
Sun, Ming
中科院分区：
文献类型：
--
作者：
Huang, Junyan;Han, Dongmei;Sun, Ming
苏云金芽孢杆菌亚种新的隐蔽表达载体pBMB175。分离并鉴定了Tenebrionis YBT-1765。序列分析表明，pBMB175(14841bp，GC含量为31%)至少含有18个开放阅读框(ORF)，其中9个ORF与滚环复制质粒pGI3中的假设蛋白质具有同源性。缺失分析表明，pBMB175小复制子位于1,151个碱基的新片段中。该片段含有ORF7编码序列，编码一种对质粒复制必不可少的蛋白质(Rep175,149个氨基酸[AA])。Rep175与已知的功能蛋白没有明显的同源性。此外，在该微型复制子片段中还发现了一个假定的双链起源(DSO)，与其他复制子的特征双链起源(DSO)没有DNA相似性。这些特征表明，pBMB175可以被放入一个新的质粒家族。
A new cryptic plasmid pBMB175 from Bacillus thuringiensis subsp. tenebrionis YBT-1765 was isolated and characterized. Sequence analysis showed that pBMB175 (14,841 bp and 31% GC content) contained at least eighteen putative open reading frames (ORFs), among which nine ORFs displayed the homology with the hypothetical proteins in rolling-circle replication plasmid pGI3. Deletion analysis revealed that the pBMB175 minireplicon located in a novel 1,151 bp fragment. This fragment contains ORF7 coding sequence, which encodes a protein (Rep175, 149 amino acids [aa]) indispensable for plasmid replication. Rep175 has no significant homology with known function proteins. Furthermore, a putative double-strand origin (dso), having no DNA similarity with characterized dso of other replicon so far, was identified in this minireplicon fragment. These features showed that pBMB175 could be placed into a new plasmid family.