Quantifying the relation between adhesion ligand-receptor bond formation and cell phenotype
Quantifying the relation between adhesion ligand-receptor bond formation and cell phenotype
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DOI：
10.1073/pnas.0605960103
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发表时间：
2006-12-05
影响因子：
11.1
通讯作者：
Mooney, David J.
中科院分区：
文献类型：
--
作者：
Kong, Hyun Joon;Boontheekul, Tanyarut;Mooney, David J.
细胞生物学中的基本相互作用之一是细胞受体与粘附配体的结合，并且细胞行为的许多方面被认为是由形成的这些键的数量调节的。不幸的是，缺乏方法来量化键的形成，特别是对于3D培养或组织中的细胞，已经排除了直接探测这一假设。我们现在证明，FRET技术可用于定量细胞受体和人工细胞外基质偶联的合成粘附寡肽之间形成的键的数量。相似的定量关系被发现之间的键数和MC 3 T3-E1前成骨细胞和C2 C12成肌细胞的增殖和分化，虽然这种关系是不同的每种细胞类型。这种理解3D细胞-细胞外基质相互作用的方法将允许人们预测细胞行为并使用键数作为合成细胞外基质的基本设计标准。
One of the fundamental interactions in cell biology is the binding of cell receptors to adhesion ligands, and many aspects of cell behavior are believed to be regulated by the number of these bonds that form. Unfortunately, a lack of methods to quantify bond formation, especially for cells in 3D cultures or tissues, has precluded direct probing of this assumption. We now demonstrate that a FRET technique can be used to quantify the number of bonds formed between cellular receptors and synthetic adhesion oligopeptides coupled to an artificial extracellular matrix. Similar quantitative relations were found between bond number and the proliferation and differentiation of MC3T3-E1 preosteoblasts and C2C12 myoblasts, although the relation was distinct for each cell type. This approach to understanding 3D cell-extracellular matrix interactions will allow one to both predict cell behavior and to use bond number as a fundamental design criteria for synthetic extracellular matrices.