Evaluation of Tat-encoding bicistronic human immunodeficiency virus type 1 gene transfer vectors in primary canine bone marrow mononuclear cells.
Evaluation of Tat-encoding bicistronic human immunodeficiency virus type 1 gene transfer vectors in primary canine bone marrow mononuclear cells.
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原代犬骨髓单核细胞中编码 Tat 的双顺反子人类免疫缺陷病毒 1 型基因转移载体的评价。
DOI：
10.1128/jvi.76.14.7334-7342.2002
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发表时间：
2002
影响因子：
5.4
通讯作者：
Schuening,Friedrich
中科院分区：
文献类型：
--
作者：
Srinivasakumar,Narasimhachar;Zaboikin,Michail;Zaboikina,Tatiana;Schuening,Friedrich
对编码人类免疫缺陷病毒1型(HIV-1)的TAT基因转移载体在原代犬骨髓单个核细胞中的表达进行了评价。与带有内部启动子的载体相比，TAT载体提供了更高水平的基因表达。在体外，HIV-1载体也比Moloney小鼠白血病病毒(MoMLV)载体更有效地转导犬骨髓单个核细胞。用转导的自体骨髓细胞进行的犬移植实验证实了HIV-1载体比MoMLV载体在将基因转移到犬骨髓细胞方面的优越性。TAT载体不仅可用于在造血细胞中提供高水平的治疗性基因表达，还可用于在犬模型中研究TAT在这些组织中的生物学效应。
Tat-encoding human immunodeficiency virus type 1 (HIV-1) gene transfer vectors were evaluated in primary canine bone marrow mononuclear cells. Tat vectors provided higher levels of gene expression than vectors with internal promoters. The HIV-1 vector was also more efficient than Moloney murine leukemia virus (MoMLV) vectors for transduction of canine bone marrow mononuclear cells in vitro. Transplantation experiments in dogs with transduced autologous marrow cells confirmed the superiority of HIV-1 vectors over MoMLV vectors for gene transfer into canine bone marrow cells. Tat vectors may be useful not only for providing high levels of therapeutic gene expression in hematopoietic cells but also for study of the biological effects of Tat in those tissues in the canine model.