A MAIZE DNA-BINDING FACTOR WITH A BZIP MOTIF IS INDUCED BY LOW-TEMPERATURE
A MAIZE DNA-BINDING FACTOR WITH A BZIP MOTIF IS INDUCED BY LOW-TEMPERATURE
复制标题
DOI：
10.1007/bf02423445
复制
发表时间：
1995-09-20
期刊：
影响因子：
--
通讯作者：
SUGAWARA, K
中科院分区：
文献类型：
--
作者：
KUSANO, T;BERBERICH, T;SUGAWARA, K
我们利用水稻lip 19与玉米进行杂交，分离到一个低温诱导的玉米基因mlip 15。分离的最长cDNA包含1179 bp，编码135个氨基酸的bZIP（碱性区/亮氨酸拉链）蛋白。该基因在DNA和氨基酸水平上与水稻基因的同源性分别为61.4%和68.9%，与其他玉米编码bZIP蛋白的基因不同。低温对mlip 15的转录水平有正向调节作用，其作用方式与lip 19相同。盐胁迫和外源脱落酸也显著提高了该基因的转录水平，而厌氧条件下则略有提高，但热激和干旱对该基因的转录水平没有影响。在兔网织红细胞裂解物或大肠杆菌表达系统中产生的mLIP 15蛋白和截短的衍生物能够与小麦组蛋白H3基因启动子的片段结合。通过添加摩尔过量的在启动子中发现的六聚体序列5 ′-ACGTCA-3 ′和G盒样序列，而不是通过添加ocs序列或突变的六聚体序列，这种结合被减弱。与玉米Adh 1基因的启动子区结合的因子，其表达也由低温诱导。这些结果导致的结论是，mlip 15是一个强有力的候选人在玉米低温诱导的转录因子。
We have isolated a low temperature-induced maize gene, mlip15, via cross hydridization using rice lip19. The longest cDNA isolated comprised 1179 bp and coded for a 135 amino acid bZIP (basic region/leucine zipper) protein. The gene showed 61.4% and 68.9% identity with the rice gene at the DNA and amino acid sequence levels, respectively, and is distinct from other maize genes that code for bZIP proteins. The level of mlip15 transcript was positively regulated by low temperature in the same way as the lip19 transcript. The levels of the transcript were also strongly increased by salt stress and exogenous abscisic acid, and slightly increased by anaerobiosis, but were not affected by heat shock and drought. The mLIP15 protein and truncated derivatives, produced in rabbit reticulocyte lysates or in an Escherichia coli expression system, were able to bind to a fragment of the wheat histone H3 gene promoter. This binding was diminished by addition of a molar excess of the hexamer sequence 5'-ACGTCA-3' found in the promoter and of the G-box-like sequence, but not by the addition of the ocs sequence or a mutated hexamer sequence. The factor bound to a promoter region of the maize Adh1 gene, expression of which is also induced by low temperature. These results lead to the conclusion that mlip15 is a strong candidate for a low temperature-induced transcription factor in maize.