Circular RNA expression profiles reveal that hsa_circ_0018289 is up-regulated in cervical cancer and promotes the tumorigenesis.
Circular RNA expression profiles reveal that hsa_circ_0018289 is up-regulated in cervical cancer and promotes the tumorigenesis.
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DOI：
10.18632/oncotarget.21257
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发表时间：
2017-10-17
期刊：
影响因子：
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通讯作者：
Cao LL
中科院分区：
文献类型：
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作者：
Gao YL;Zhang MY;Xu B;Han LJ;Lan SF;Chen J;Dong YJ;Cao LL
环状RNAs(CircRNAs)是一类非编码RNAs，已被认为是多种疾病，特别是癌症的关键调控因子。然而，CircRNAs在宫颈癌中的表达谱和功能仍不清楚。本研究利用人CircRNAs微阵列技术筛选宫颈癌组织中CircRNAs的表达。微阵列分析发现45个CircRNAs有4倍的变化。在这些上调的CircRNA中，hsa_CIRC_0018289在35对宫颈癌组织中显著上调，与癌旁正常组织和细胞系相比。功能丧失实验表明，体内外hSA_CIRC_0018289基因敲除可抑制宫颈癌细胞的增殖、迁移和侵袭。通过生物信息学预测程序和荧光素酶报告基因分析，观察到hSA_CIRC_0018289与miR-497直接结合。综上所述，hSA_CIRC_0018289在宫颈癌的增殖、转移和侵袭中起重要作用，提示hSA_CIRC_0018289的miRNA“海绵”及其在宫颈癌发生中的致癌作用。
Circular RNAs (circRNAs) are a type of non-coding RNAs that have been identified as critical regulators in various diseases, especially in cancers. However, the expression profiles and functions of circRNAs in cervical cancer are still unclear. In present study, human circRNAs microarray were performed to screen the circRNAs expression in cervical cancer tissue. Microarray analysis revealed 45 significantly expressed circRNAs with 4 fold change. Among these up-regulated circRNAs, hsa_circ_0018289 was validated to be significantly up-regulated in 35 pairs of cervical cancer tissue compared with adjacent normal tissue and cell lines. Loss-of-function experiments revealed that, in vitro and in vivo, hsa_circ_0018289 knockdown inhibited the proliferation, migration and invasion of cervical cancer cells. Via bioinformatics prediction program and luciferase reporter assays, hsa_circ_0018289 was observed to directly bind to miR-497. Taken together, the results indicate that hsa_circ_0018289 plays important role in cervical cancer proliferation, migration and invasion, suggesting the miRNA ‘sponge’ of hsa_circ_0018289 and its oncogenic role on cervical cancer tumorigenesis.