Simple in vitro migration assay for neural crest cells and the opposite effects of all-trans-retinoic acid on cephalic- and trunk-derived cells.
Simple in vitro migration assay for neural crest cells and the opposite effects of all-trans-retinoic acid on cephalic- and trunk-derived cells.
复制标题
神经嵴细胞的简单体外迁移测定以及全反式视黄酸对头源性和躯干源性细胞的相反作用。
作者：
Usami M;Mitsunaga K;Irie T;Miyajima A;Doi O.
在这里，我们描述了一个简单的体外神经嵴细胞（NCC）迁移试验和全反式维甲酸（RA）对NCC的影响。将从10.5天大鼠胚胎的菱形脑或躯干区域切除的神经管培养48小时，以允许NCC的迁出和迁移。NCC的迁移被测量为从培养24和48小时之间的NCC的圆形散布计算的半径的变化（半径比）。24 h后，将RA加入培养基中，通过大鼠全胚胎培养确定胚胎毒性浓度。RA（10 μM）抑制头侧NCC的迁移，而促进躯干侧NCC的迁移，表明RA对这两种类型的NCC有相反的作用。
Here, we describe a simplein vitroneural crest cell (NCC) migration assay and the effects of all‐trans‐retinoic acid (RA) on NCCs. Neural tubes excised from the rhombencephalic or trunk region of day 10.5 rat embryos were cultured for 48 h to allow emigration and migration of NCCs. Migration of NCCs was measured as the change in the radius (radius ratio) calculated from the circular spread of NCCs between 24 and 48 h of culture. RA was added to the culture medium after 24 h at embryotoxic concentrations determined by rat whole embryo culture. RA (10 μM) reduced the migration of cephalic NCCs, whereas it enhanced the migration of trunk NCCs, indicating that RA has opposite effects on these two types of NCCs.