New Hosts of The Lassa Virus.
New Hosts of The Lassa Virus.
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DOI：
10.1038/srep25280
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发表时间：
2016-05-03
影响因子：
4.6
通讯作者：
Fichet-Calvet E
中科院分区：
文献类型：
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作者：
Olayemi A;Cadar D;Magassouba N;Obadare A;Kourouma F;Oyeyiola A;Fasogbon S;Igbokwe J;Rieger T;Bockholt S;Jérôme H;Schmidt-Chanasit J;Garigliany M;Lorenzen S;Igbahenah F;Fichet JN;Ortsega D;Omilabu S;Günther S;Fichet-Calvet E
拉沙病毒（LASV）在人类中引起致命的出血热，每年在西非造成数千人死亡。40年来，纳塔尔多哺乳动物大鼠，Mastomys natalensis，一直被认为是LASV的唯一宿主。我们发现证据表明LASV也由其他啮齿动物物种宿主：尼日利亚的非洲林鼠Hylomyscus pamfi，以及尼日利亚和几内亚的几内亚多乳鼠Mastomys erythroleucus。在BSL-4实验室中分离这些动物的病毒株并进行完全测序。对编码糖蛋白、核蛋白、聚合酶和基质蛋白的病毒基因的系统发育分析表明，在M. erythroleucus属于谱系III和IV。从H. pamfi聚类在谱系I附近（对于S基因）和II与III之间（对于L基因）。新的啮齿类宿主的发现对LASV的进化及其向西非新地区的传播具有重要意义。
Lassa virus (LASV) causes a deadly haemorrhagic fever in humans, killing several thousand people in West Africa annually. For 40 years, the Natal multimammate rat, Mastomys natalensis, has been assumed to be the sole host of LASV. We found evidence that LASV is also hosted by other rodent species: the African wood mouse Hylomyscus pamfi in Nigeria, and the Guinea multimammate mouse Mastomys erythroleucus in both Nigeria and Guinea. Virus strains from these animals were isolated in the BSL-4 laboratory and fully sequenced. Phylogenetic analyses of viral genes coding for glycoprotein, nucleoprotein, polymerase and matrix protein show that Lassa strains detected in M. erythroleucus belong to lineages III and IV. The strain from H. pamfi clusters close to lineage I (for S gene) and between II & III (for L gene). Discovery of new rodent hosts has implications for LASV evolution and its spread into new areas within West Africa.