Novel antihypertensives targeted at dopamine beta-monooxygenase: turnover-dependent cofactor depletion by phenyl aminoethyl selenide.
Novel antihypertensives targeted at dopamine beta-monooxygenase: turnover-dependent cofactor depletion by phenyl aminoethyl selenide.
复制标题
针对多巴胺β-单加氧酶的新型抗高血压药：苯氨乙基硒化物导致周转依赖性辅因子消耗。
DOI：
10.1021/jm00401a003
复制
发表时间：
1988
影响因子：
7.3
通讯作者：
Pollock,SH
中科院分区：
文献类型：
--
作者：
May,SW;Wimalasena,K;Herman,HH;Fowler,LC;Ciccarello,MC;Pollock,SH
反应混合物含有1.03 mM抗坏血酸盐和标准DBM测定混合物的其它组分（参考文献11），并已用100%O2（约100 mM）预平衡。1.1通过添加底物至所示的最终浓度来引发每个反应，然后监测反应混合物中的溶解氧浓度。酶耗氧终止后，取出各反应混合物的等分试样并通过HPLC进行分析，以定量底物、产物和抗坏血酸盐。6由于反应混合物中产物的浓度非常低，产物峰几乎不可见，并且通过HPLC难以分辨。c在取出样品用于定量底物、产物和抗坏血酸盐之后，将反应混合物用100%O2再平衡，并再次加入抗坏血酸盐至终浓度为1.0 mM。
0Initial [substrate], 0.25 mM. Reaction mixtures contained 1.03 mM ascorbate and other components of the standard DBM assay mixture (ref 11) and had been preequilibrated with 100% 02 (ca. 1.1 mM) for 10 min. Each reaction was initiated by addition of substrate to the final concentration indicated and was then followed by monitoring the dissolved oxygen concentration in the re-action mixture. Upon termination of enzymatic oxygen consump-tion, aliquots of each reaction mix were withdrawn and analyzed by HPLC in order to quantitate substrate, product, and ascorbate. 6 Due to the very low concentrationof product in the reaction mix, the product peak was barely visible and was poorly resolved by HPLC. c After samples were withdrawn for quantitation of substrate, product, and ascorbate, the reaction mixtures were reequi-librated with 100% 02, and ascorbate was again added to a final concentration of 1.0 mM.