Development of a near-isogenic line population of Arabidopsis thaliana and comparison of mapping power with a recombinant inbred line population
Development of a near-isogenic line population of Arabidopsis thaliana and comparison of mapping power with a recombinant inbred line population
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DOI：
10.1534/genetics.106.066423
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发表时间：
2007-02-01
期刊：
影响因子：
3.3
通讯作者：
Koornneef, Maarten
中科院分区：
文献类型：
--
作者：
Keurentjes, Joost J. B.;Bentsink, Leonie;Koornneef, Maarten
在拟南芥中，重组自交系（RIL）群体被广泛用于数量性状位点（QTL）分析。然而，这类群体的定位分析可能受到限制，因为主效QTL的掩蔽效应和多个QTL的上位性相互作用。在植物物种中常用的另一种类型的永生实验群体是渐渗系组。在这里，我们介绍了一个全基因组覆盖近等基因系（NIL）人口的拟南芥的发展，从佛得角群岛（Cvi）加入基因组区域渗入到兰茨贝格直立（Ler）的遗传背景。我们已经经验比较了这个新的人口与已经存在的RIL人口来自相同的父母QTL定位能力。为此，我们分析并定位了影响6个遗传力不同的发育性状的QTL。总体而言，在近等基因系群体中，虽然定位分辨率较低，但可以检测到比RIL群体小的效应QTL。此外，我们估计了群体大小和重复数对影响发育性状的QTL检测能力的影响。一般来说，群体大小比重复的数量更重要，以提高RIL的定位能力，而对于NIL，几个重复是绝对必要的。这些分析有助于利用这两种常见的分离群体进行QTL定位的实验设计。
In Arabidopsis recombinant inbred line (RIL) populations are widely used for quantitative trait locus (QTL) analyses. However, mapping analyses with this type of population can be limited because of the masking effects of major QTL and epistatic interactions of multiple QTL. An alternative type of immortal experimental population commonly used in plant species are sets of introgression lines. Here we introduce the development of a genomewide coverage near-isogenic line (NIL) population of Arabidopsis thaliana, by introgressing genomic regions from the Cape Verde Islands (Cvi) accession into the Landsberg erecta (Ler) genetic background. We have empirically compared the QTL mapping power of this new population with an already existing RIL population derived from the same parents. For that, we analyzed and mapped QTL affecting six developmental traits with different heritability. Overall, in the NIL population smaller-effect QTL than in the RIL population could be detected although the localization resolution was lower. Furthermore, we estimated the effect of population size and of the number of replicates on the detection power of QTL affecting the developmental traits. In general, population size is more important than the number of replicates to increase the mapping power of RILs, whereas for NILs several replicates are absolutely required. These analyses are expected to facilitate experimental design for QTL mapping using these two common types of segregating populations.