The anti-biofilm and collagen-stabilizing effects of proanthocyanidin as an auxiliary endodontic irrigant
The anti-biofilm and collagen-stabilizing effects of proanthocyanidin as an auxiliary endodontic irrigant
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原花青素作为辅助牙髓冲洗剂的抗生物膜和胶原稳定作用
DOI：
10.1111/iej.13280
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发表时间：
2020
影响因子：
5
通讯作者：
Gong SQ
中科院分区：
文献类型：
--
作者：
Yang SY;Liu Y;Mao J;Wu YB;Deng YL;Qi SC;Zhou YC;Gong SQ
目的探讨原花青素（PA）溶液对粪肠球菌（Enterococcus faecalis，E. faecalis）的成膜作用及其对脱矿根管牙本质力学性能和生物降解抗力的影响。用以下冲洗液处理感染1周龄粪肠球菌生物膜的牙本质块：无菌水（对照）、2%洗必泰（CHX）、2% PA、5% PA和10% PA。处理后，使用共聚焦激光扫描显微镜分析粪肠球菌生物膜内的活菌和死菌比例。为了评价经上述冲洗剂处理的完全脱矿牙本质的生物稳定性，分别在基线、冲洗剂处理后和生物降解后测试在胶原酶溶液中孵育的人牙本质的弹性模量和羟脯氨酸释放。此外，通过X射线光电子能谱（XPS）表征经各种冲洗剂处理的脱矿牙本质胶原的表面化学键。结果PA组和CHX组粪肠球菌死亡率明显高于对照组（P<0. 05）。PA灌洗可显著提高脱矿牙本质的力学性能（P< 0.05），且随PA浓度的增加而增强。CHX组和PA组的弹性损失和羟脯氨酸释放量显著低于对照组（P< 0.05）。结果表明，PA对牙本质胶原的生物改性作用可通过增加C1 s下的C-O/C-N峰百分比和O 1 s窄扫描XPS下的C-O峰百分比来证实。它可以用作辅助性根管冲洗剂，具有保护膜和胶原稳定作用。
AimTo evaluate the antibiofilm effect of proanthocyanidin (PA) solution as an irrigant againstEnterococcus faecalis (E. faecalis)and its influence on the mechanical properties and biodegradation resistance of demineralized root dentine.MethodologyEnterococcus faecaliswere introduced into human root dentine tubules by a serial centrifugation method and grown for 1 week. Dentine blocks infected with 1‐week‐oldE. faecalisbiofilms were treated with the following irrigants: sterile water (control), 2% chlorhexidine (CHX), 2% PA, 5% PA and 10% PA. After treatment, the live and dead bacteria proportions withinE. faecalisbiofilms were analysed using confocal laser scanning microscopy. To evaluate the biostability of fully demineralized dentine treated by the aforementioned irrigants, the elastic modulus and hydroxyproline release of human dentine incubated in collagenase solution were tested at baseline, after irrigant treatment and after biodegradation, respectively. Furthermore, the surface chemical bond of demineralized dentine collagen treated by various irrigants was characterized by X‐ray photoelectron spectroscopy (XPS). Statistical analysis was performed using one‐wayanovaand Tukey’spost hocmultiple comparisons with the significance level at 5%.ResultsThe proportion of deadE. faecalisvolume was significantly higher in the PA and CHX groups than that in the control group (P< 0.05). PA irrigation significantly increased the mechanical properties of demineralized dentine (P< 0.05), and the effect was enhanced with increasing PA concentration. CHX and PA groups had significantly less elasticity loss and hydroxyproline release (P< 0.05). The biomodification of dentine collagen by PA was verified by increased C‐O/C‐N peak percentage under C1s and C‐O peak percentage under O1s narrow‐scan XPS spectra.ConclusionsProanthocyanidin killedE. faecaliswithin biofilms and enhanced the biostability of the collagen matrix of demineralized root dentine. It might be used as an auxiliary endodontic irrigant with antibiofilm and collagen‐stabilizing effects.