Influence of a Roundup formulation on glyphosate effects on steroidogenesis and proliferation of bovine granulosa cells in vitro
Influence of a Roundup formulation on glyphosate effects on steroidogenesis and proliferation of bovine granulosa cells in vitro
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DOI：
10.1016/j.chemosphere.2017.09.007
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发表时间：
2017-12-01
期刊：
影响因子：
8.8
通讯作者：
Spicer, Leon J.
中科院分区：
文献类型：
--
作者：
Perego, Maria Chiara;Caloni, Francesca;Spicer, Leon J.
甘氨酰（N-膦酰甲基-甘氨酸）是一种非选择性内吸除草剂，在世界范围内广泛使用。本研究的目的是确定单独使用草甘膦（GLPH）或草甘膦与农达（G-RU）的制剂是否会影响颗粒细胞增殖和类固醇产生。进行了四个实验。In 'Exp. 1、10和300 μ g/mL的GLPH对细胞数量、雌二醇或孕酮的产生没有影响（P > 0.05），而10和300 μ g/mL的G-RU显著降低（P < 0.05）细胞数量和雌二醇和孕酮的产生。在Exp. G-RU浓度为0.1 μ g/mL时，对GC数、孕酮和雌二醇的产生无显著影响（P> 0.05），而G-RU浓度为10 μ g/mL时，对GC数、孕酮和雌二醇的产生有显著影响（P <0.05）。在不存在IGF 1但存在FSH的情况下，1 μ g/mL的G-RU降低（P < 0.05）雌二醇的产生，而在存在IGF 1和FSH的情况下，1 μ g/mL的G-RU增加（P < 0.05）细胞数量、孕酮和雌二醇的产生。在Exp. 3，IGF 1显著增加细胞数量（2.8倍）和雌二醇（17.8倍）和孕酮（6.1倍）的产生。单独10 μ g/mL的GLPH对FSH诱导的（即，基础）或FSH加IGF 1诱导的细胞数、雌二醇或孕酮产生。然而，10 μ g/mL的G-RU显著抑制FSH加IGF 1诱导的细胞数量、雌二醇和孕酮的产生，抑制率为65%-91%。在Exp. G-RU处理4、48 h对贴壁细胞活力无明显影响。总之，目前的研究表明，GLPH，特别是G-RU可能会损害牛的生殖功能。(C)2017爱思唯尔有限公司版权所有
Glyphosate (N-phosphonomethyl-glycine) is a non-selective systemic herbicide widely used worldwide. The purpose ofthis study is to determine if glyphosate alone (GLPH) or in formulation with Roundup (G-RU) can affect granulosa cell proliferation and steroid production. Four experiments were conducted. In 'Exp. 1, 10 and 300 mu g/mL of GLPH had no effect (P > 0.05) on cell numbers, estradiol or progesterone production, whereas 10 and 300 mu g/mL of G-RU dramatically decreased (P < 0.05) cell numbers and estradiol and progesterone production. In Exp. 2, G-RU at 0.1 mu g/mL had no significant effect whereas G-RU at 10 mu g/mL decreased (P < 0:05) GC numbers, progesterone and estradiol production. In the absence of IGF1 but presence of FSH, 1 mu g/mL of G-RU decreased (P < 0.05) estradiol production, whereas in the presence of IGF1 and FSH, 1 mu g/mL of G-RU increased (P < 0.05) cell numbers, progesterone and estradiol production. In Exp. 3, IGF1 significantly increased cell numbers (by 2.8-fold) and estradiol (by 17.8-fold) and progesterone (by 6.1-fold) production. GLPH at 10 mu g/mL alone had no significant effect on FSH-induced (i.e., basal) or FSH plus IGF1-induced cell numbers, estradiol or progesterone production. However, G-RU at 10 mu g/mL significantly inhibited FSH plus IGF1-induced cell numbers, estradiol and progesterone production by 65%-91%. In Exp. 4, 48 h treatment of G-RU had no significant effect on viability of attached cells. In conclusion, the present studies demonstrate that GLPH and particularly G-RU may have the potential to impair reproductive function in cattle. (C) 2017 Elsevier Ltd. All rights reserved.