SNC1, A YEAST HOMOLOG OF THE SYNAPTIC VESICLE-ASSOCIATED MEMBRANE-PROTEIN SYNAPTOBREVIN GENE FAMILY - GENETIC INTERACTIONS WITH THE RAS AND CAP GENES
SNC1, A YEAST HOMOLOG OF THE SYNAPTIC VESICLE-ASSOCIATED MEMBRANE-PROTEIN SYNAPTOBREVIN GENE FAMILY - GENETIC INTERACTIONS WITH THE RAS AND CAP GENES
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DOI：
10.1073/pnas.89.10.4338
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发表时间：
1992-05-15
影响因子：
11.1
通讯作者：
WIGLER, M
中科院分区：
文献类型：
--
作者：
GERST, JE;RODGERS, L;WIGLER, M
SNC 1是来自酿酒酵母的基因，编码脊椎动物突触囊泡相关膜蛋白（VAMP）或突触小泡蛋白的同源物。SNC 1通过其抑制S.具有激活的RAS 2等位基因的酿酒酵母菌株。CAP是RAS响应S.酿酒酵母腺苷酸环化酶复合物。CAP的N-末端结构域是细胞对活化的RAS蛋白的完全反应所必需的。CAP的C-末端结构域是正常细胞形态和对营养极端反应所必需的。表达SNC 1的多拷贝质粒仅抑制CAP的C-末端功能的丧失，并且仅在存在活化的RAS 2的情况下。
SNC1, a gene from the yeast Saccharomyces cerevisiae, encodes a homolog of vertebrate synaptic vesicle-associated membrane proteins (VAMPs) or synaptobrevins. SNC1 was isolated by its ability to suppress the loss of CAP function in S. cerevisiae strains possessing an activated allele of RAS2. CAP is a component of the RAS-responsive S. cerevisiae adenylyl cyclase complex. The N-terminal domain of CAP is required for full cellular responsiveness to activated RAS proteins. The C-terminal domain of CAP is required for normal cellular morphology and responsiveness to nutrient extremes. Multicopy plasmids expressing SNC1 suppress only the loss of the C-terminal functions of CAP and only in the presence of activated RAS2.