Characterization and optimization of production of exopolysaccharide from Chlamydomonas reinhardtii
Characterization and optimization of production of exopolysaccharide from Chlamydomonas reinhardtii
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DOI：
10.1016/j.carbpol.2013.02.016
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发表时间：
2013-06-20
影响因子：
11.2
通讯作者：
Bafana, Amit
中科院分区：
文献类型：
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作者：
Bafana, Amit
莱茵衣单胞菌（Chlamydomonas reinhardtii）菌株RAC具有分泌大量胞外多糖（EPS）的能力。纯化后的EPS分子量为2.25 × 10(5) Da，呈纤维状结构，表面呈片状。化学分析显示含有半乳糖醛酸、核糖、阿拉伯糖、木糖、葡萄糖、半乳糖和鼠李糖。采用经典的单次法和Plackett-Burman设计优化EPS的生产，然后采用响应面法优化EPS的生产。得到的响应面模型具有统计学意义（p< 0.5），预测EPS最大产量为628 mg/L。最佳生产培养基为CaCl2 - 74、NaNO3 - 422、K-2 HPO4 - 10和MgSO4 - 200 mg/L， pH为7。EPS具有显著的抗氧化活性，具有一定的工业应用价值。这是首次报道从印度分离的衣单胞菌菌株的特性和生产EPS。讨论了其与先前报道的EPS的差异。(C) 2013 Elsevier Ltd.版权所有。
Chlamydomonas reinhardtii strain RAC was isolated based on its ability to secrete large amount of exopolysaccharide (EPS). The purified EPS had a molecular weight of 2.25 x 10(5) Da, and showed fibrillar structure with surfaces having sheet-like appearance. Chemical analysis showed the presence of galacturonic acid, ribose, arabinose, xylose, glucose, galactose and rhamnose sugars. The production of EPS was optimized by the classical one-at-a-time approach and Plackett-Burman design, followed by response surface methodology. The resulting response surface model was statistically significant (p< 0.5) and predicted maximum EPS production of 628 mg/L. The optimum production medium consisted of CaCl2 - 74, NaNO3 - 422, K-2 HPO4 - 10 and MgSO4 - 200 mg/L with a pH 7. The EPS showed significant antioxidant activity, which can have several industrial applications. This is the first report on characterization and production of EPS from a Chlamydomonas strain isolated from India. Its differences from the earlier reported EPS are discussed. (C) 2013 Elsevier Ltd. All rights reserved.