Adhesion kinetics of functionalized vesicles and mammalian cells: A comparative study
Adhesion kinetics of functionalized vesicles and mammalian cells: A comparative study
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DOI：
10.1021/la0261747
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发表时间：
2003-03-04
期刊：
影响因子：
3.9
通讯作者：
Wegener, J
中科院分区：
文献类型：
--
作者：
Reiss, B;Janshoff, A;Wegener, J
石英晶体微天平技术（QCM）的适用性，以监测细胞-基板接触的形成和调制在真实的时间最近已经建立。一个更详细的分析QCM响应时，活细胞附着和传播的谐振器表面上，但是，阻碍了细胞系统的化学和机械的复杂性和实验的困难，以控制一个单一的参数细胞基板接触在一个可预测的方式。在这项研究中，我们利用脂质体作为简单的细胞模型，并研究了这些脂质体与谐振器表面的相互作用。为了模拟细胞和蛋白质包被的基板之间的特定的相互作用，在细胞培养实验中给出的，我们将生物素标记的脂质作为“受体”的脂质体外壳和预吸附的抗生物素蛋白的谐振器表面上。应用耗散QCM（D-QCM）技术监测脂质体吸附过程中共振频率和能量耗散的变化。我们还研究了吸附动力学的脂质体掺杂的生物素部分连接到脂质核心的烷基间隔，以增加脂质体壳和谐振器表面之间的距离。这些数据的比较与哺乳动物细胞的粘附动力学监测D-QCM提出和讨论。虽然谐振频率的变化对于完整的脂质体和哺乳动物细胞非常相似，但是当细胞附着并在谐振器表面上扩散时，粘性能量耗散显著更高。
The suitability of the quartz crystal microbalance technique (QCM) to monitor the formation and modulation of cell- substrate contacts in real time has recently been established. A more detailed analysis of the QCM response when living cells attach and spread on the resonator surfaces is, however, hampered by the chemical and mechanical complexity of cellular systems and the experimental difficulties to control one single parameter of cell-substrate contacts in a predictable way. In this study, we made use of liposomes as simple cell models and studied the interactions of these liposomes with the resonator surface. To mimic the specific interactions between cell and protein-coated substrate as given in cell culture experiments, we incorporated biotin-labeled lipids as "receptors" in the liposome shell and preadsorbed avidin on the resonator surface. The dissipational QCM (D-QCM) technology was applied to monitor the shifts in resonance frequency and energy dissipation during the adsorption of liposomes prepared with increasing amounts of biotin-labeled lipids. We also studied the adsorption kinetics of liposomes doped with biotin moieties that were attached to the lipid core by an alkyl spacer in order to increase the distance between liposome shell and resonator surface. A comparison of these data with the adhesion kinetics of mammalian cells as monitored by D-QCM is presented and discussed. Although the shifts in resonance frequency are very similar for intact liposomes and mammalian cells, the viscous energy dissipation is significantly higher when cells attach and spread on the resonator surface.