SEPARATE NEURONAL POPULATIONS OF THE RAT GLOBUS-PALLIDUS PROJECTING TO THE SUBTHALAMIC NUCLEUS, AUDITORY-CORTEX AND PEDUNCULOPONTINE TEGMENTAL AREA
SEPARATE NEURONAL POPULATIONS OF THE RAT GLOBUS-PALLIDUS PROJECTING TO THE SUBTHALAMIC NUCLEUS, AUDITORY-CORTEX AND PEDUNCULOPONTINE TEGMENTAL AREA
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DOI：
10.1016/0306-4522(92)90156-v
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发表时间：
1992-02-01
期刊：
影响因子：
3.3
通讯作者：
HATTORI, T
中科院分区：
文献类型：
--
作者：
MORIIZUMI, T;HATTORI, T
用逆行荧光示踪剂研究了大鼠苍白球神经元向丘脑下核、听觉皮层和桥脚被盖核发送轴突的地形分布。投射到丘脑下核的神经元位于苍白球的吻侧，而投射到听觉皮层和桥脚被盖核的神经元位于尾侧。在尾端苍白球内，皮层神经元和被皮层神经元也以不同的方式分布。前者神经元较大且位于腹内侧，后者神经元中等大小且位于背外侧。采用逆行荧光示踪技术结合胆碱乙酰转移酶免疫荧光组化，发现绝大多数皮层神经元表达胆碱乙酰转移酶免疫反应性，而皮层神经元很少表现出胆碱乙酰转移酶免疫反应性。小麦胚芽凝集素结合辣根过氧化物酶的逆行示踪方法与谷氨酸脱羧酶的免疫组织化学相关，证实了白斑被盖通路的gaba能性质。本研究揭示了投射到丘脑底核、听觉皮层和桥脚被盖核的苍白球神经元的独立性。在这种细胞排列中，还发现在尾侧苍白质水平存在功能不同的神经元群，大的胆碱能细胞支配新皮层，中等的氨基丁酸能细胞“喂养”中脑被盖。
The topographic arrangement of globus pallidus neurons sending axons to the subthalamic nucleus, auditory cortex and pedunculopontine tegmental nucleus was studied in the rat using retrograde fluorescent tracers. Neurons projecting to the subthalamic nucleus were localized in the rostral part of the globus pallidus, while neurons projecting to the auditory cortex and to the pedunculopontine tegmental nucleus were located in the caudal part. The two populations of pallidocortical and pallidotegmental neurons were also distributed in a separate manner within the caudal globus pallidus. The former neurons were large and located more ventromedially, whereas the latter were medium-sized and located more dorsolaterally. Using a retrograde fluorescent tracing technique combined with choline acetyltransferase immunofluorescence histochemistry, it was found that a vast majority of pallidocortical neurons expressed choline acetyltransferase immunoreactivity, and that pallidotegmental neurons rarely exhibited choline acetyltransferase immunoreactivity. A method of retrograde tracing with wheatgerm agglutinin conjugated with horseradish peroxidase associated to immunohistochemistry for glutamate decarboxylase confirmed the GABAergic nature of the pallidotegmental pathway.The present study revealed the independent nature of the globus pallidus neurons projecting to the subthalamic nucleus, auditory cortex and pedunculopontine tegmental nucleus. Within this cellular arrangement, the presence of functionally distinct neuronal populations at the caudal pallidal level was also identified, with large cholinergic cells innervating the neocortex and medium-sized GABAergic cells "feeding" the mesencephalic tegmentum.