GLUCOSE-METABOLISM OF ISOLATED ECCRINE SWEAT GLAND .2. RELATION BETWEEN GLUCOSE-METABOLISM AND SODIUM-TRANSPORT
GLUCOSE-METABOLISM OF ISOLATED ECCRINE SWEAT GLAND .2. RELATION BETWEEN GLUCOSE-METABOLISM AND SODIUM-TRANSPORT
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DOI：
10.1172/jci107401
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发表时间：
1973-01-01
影响因子：
15.9
通讯作者：
DOBSON, RL
中科院分区：
文献类型：
--
作者：
SATO, K;DOBSON, RL
本文试图进一步澄清的特点，甲胆碱或肾上腺素刺激的葡萄糖代谢的猴子外泌汗腺，特别强调其关系增加钠转运。甲胆碱或肾上腺素刺激的葡萄糖代谢（通过乳酸或14CO2的产生或两者的产生来估计）仅见于分泌曲，而不在导管中。在缺乏葡萄糖、Na+或K+的情况下，它被显著抑制。它被哇巴因（10 − 3 M）抑制，并在低钠（40 mM）、高钾（100 mM）培养基中部分抑制。2，4-二硝基苯酚（10 − 4 M）逆转哇巴因诱导的乳酸和14 CO2产生抑制，但仅部分逆转Na ++ K+剥夺诱导的抑制，表明哇巴因的代谢抑制继发于钠转运抑制。甲胆碱与肾上腺素无协同作用。乙酰唑胺（Diamox）或不含HCO3 −的培养基没有任何显著的抑制作用，这表明H+转运在汗腺功能中可能不重要。与Wolfe等人的报告相反，人类外泌汗腺显示出相当大的氧化活性（14CO2产生量为0.42 - 0.72 nmol/腺体/h）。这些意见进行了讨论，在汗腺能量代谢和钠转运之间的联系。
This paper attempts to further clarify the characteristics of Mecholyl- or epinephrine-stimulated glucose metabolism in the isolated monkey eccrine sweat gland with special emphasis on its relationship to increased sodium transport. The Mecholyl- or epinephrine-stimulated glucose metabolism (as estimated by either lactate or14CO2production or both) is seen only in the secretory coil and not in the duct. It is markedly suppressed in the absence of glucose, Na+, or K+. It is inhibited by ouabain (10−3M) and partially suppressed in a low-sodium (40 mM), high-potassium (100 mM) medium.2,4-dinitrophenol (10−4M) reverses ouabain-induced inhibition of lactate and14CO2production but only partially reverses inhibition induced by Na++ K+deprivation, indicating that metabolic inhibition by ouabain is secondary to the inhibition of sodium transport. There is no synergism between Mecholyl and epinephrine. The absence of any significant inhibitory effects by acetazolamide (Diamox) or HCO3−-free media suggests that H+transport may not be important in sweat gland function. In contrast to a report by Wolfe et al., human eccrine sweat glands show considerable oxidative activity (14CO2production of 0.42-0.72 nmol/gland/h). These observations are discussed in terms of the linkage between sweat gland energy metabolism and sodium transport.