Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7 Salmonella, and Shigella for PulseNet
Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7 Salmonella, and Shigella for PulseNet
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DOI：
10.1089/fpd.2006.3.59
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发表时间：
2006-03-01
影响因子：
2.8
通讯作者：
Barrett, Timothy J.
中科院分区：
文献类型：
--
作者：
Ribot, Efrain M.;Fair, M. A.;Barrett, Timothy J.
标准化的快速脉冲场凝胶电泳（PFGE）协议的大肠杆菌O 157：H7亚型，沙门氏菌血清型，志贺菌属的描述。这些协议被PulseNet实验室使用，PulseNet是一个州和地方卫生部门的网络，以及其他公共卫生实验室，对这些细菌性食源性病原体进行实时PFGE亚型分析，以进行监测和疫情调查。这些方案的开发和标准化包括对试剂和反应条件的彻底优化，以确保方案在所有参与PulseNet的实验室中产生一致的结果和高质量的PFGE图谱数据。这些快速PFGE方案基于疾病控制和预防中心开发的最初3-4天标准化程序，该程序于1996年和1997年由8个独立实验室验证。通过使用这些快速标准化的PFGE协议，PulseNet实验室能够在大约24小时内对食源性病原体进行分型，从而能够早期发现食源性疾病病例群，并通常有助于确定感染的来源。
Standardized rapid pulsed-field gel electrophoresis (PFGE) protocols for the subtyping of Escherichia coli O157:H7, Salmonella serotypes, and Shigella species are described. These protocols are used by laboratories in PulseNet, a network of state and local health departments, and other public health laboratories that perform real-time PFGE subtyping of these bacterial foodborne pathogens for surveillance and outbreak investigations. Development and standardization of these protocols consisted of a thorough optimization of reagents and reaction conditions to ensure that the protocols yielded consistent results and high-quality PFGE pattern data in all the PulseNet participating laboratories. These rapid PFGE protocols are based on the original 3-4-day standardized procedure developed at Centers for Disease Control and Prevention that was validated in 1996 and 1997 by eight independent laboratories. By using these rapid standardized PFGE protocols, PulseNet laboratories are able to subtype foodborne pathogens in approximately 24 h, allowing for the early detection of foodborne disease case clusters and often aiding in the identification of the source responsible for the infections.