Effects of growth hormone treatment on serum levels of insulin-like growth factors (IGFs) and IGF binding proteins 1-4 in postmenopausal women
Effects of growth hormone treatment on serum levels of insulin-like growth factors (IGFs) and IGF binding proteins 1-4 in postmenopausal women
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DOI：
10.1046/j.1365-2265.1998.00606.x
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发表时间：
1998-12-01
影响因子：
3.2
通讯作者：
Flyvbjerg, A
中科院分区：
文献类型：
--
作者：
Kassem, M;Brixen, K;Flyvbjerg, A
背景和目的胰岛素样生长因子结合蛋白（IGFBP）调节胰岛素样生长因子（IGF）的作用和生物利用度，然而，它们在体内的调节尚不完全清楚。在这项研究中，我们研究了不同剂量的生长激素（GH）对 IGF 和 IGFBP 循环水平的影响，设计该研究是双盲和安慰剂对照的。患者接受剂量为 0.05、0.10 或 0.20 IU/kg/天的 GH 安慰剂治疗一周。 患者 40 名年龄 52-73 岁、骨量低的绝经后妇女。 测量 通过 RIA 测量血清 IGF-I 和 IGF-II，通过蛋白质印迹 (WLB) 测量 IGFBP-1-3，并与特异性抗体测定结果进行比较免疫分析中，IGFBP-4 单独通过 WLB 进行测量。结果 GH 治疗期间 IGF-I (P < 0.001) 和 IGF-II (P < 0.01) 均显着增加。此外，IGFBP-1 (P < 0.001) 和 IGFBP-2 (P < 0.001) 显着降低，而 IGFBP-3 (P < 0.001) 和 IGFBP-4 (P < 0.05) 均以剂量依赖性方式增加。逐步（向后）多元回归分析表明，IGF-I和IGF-II的变化以及年龄与血清IGFBP-1的变化相关，GH剂量、IGF-II的增加和体重指数均与IGFBP-2的减少相关，此外，血清IGF-I、IGF-II和三碘甲状腺原氨酸的增加与IGFBP-3的增加相关，此外，GH剂量与IGFBP-2的增加相关。结论GH显着升高绝经后妇女血清IGF-I、IGF-II、IGFBP-3和IGFBP-4，降低血清IGFBP-1和IGFBP-2。
BACKGROUND AND OBJECTIVE Insulin-like growth factor binding proteins (IGFBPs) modulate the actions and bioavailability of insulin-like growth factors (IGFs), however, their regulation in vivo is incompletely understood. In this study we investigated the effects of different doses of growth hormone (GH) on circulating levels of IGFs and IGFBPs,DESIGN The study was double-blind and placebo-controlled. Patients were treated with either GH in doses of 0.05, 0.10, or 0.20 IU/kg/day of placebo for one week.PATIENTS Forty post-menopausal women aged 52-73 years with low bone mass.MEASUREMENTS Serum IGF-I and IGF-II were measured by RIA while IGFBP-1-3 were measured by Western ligand blot (WLB) and compared with determinations by specific immunoassays, IGFBP-4 was measured by WLB alone.RESULTS Both IGF-I (P < 0.001) and IGF-II (P < 0.01) increased significantly during GH treatment. Additionally, IGFBP-1 (P < 0.001) and IGFBP-2 (P < 0.001) decreased significantly while IGFBP-3 (P < 0.001) and IGFBP-4 (P < 0.05) increased all in a dose-dependent manner. Stepwise (backwards) multiple regression analyses showed that the changes in IGF-I and IGF-II, and age correlated with the change in serum IGFBP-1, Both GH-dosage, the increase in IGF-II, and body mass index correlated with the decrease in IGFBP-2, Furthermore, the increase in serum IGF-I, IGF-II, and triiodothyronine correlated with the increase in IGFBP-3, Moreover, GH-dosage correlated with the increase in serum IGFBP-4,CONCLUSION GH significantly increased serum IGF-I, IGF-II, IGFBP-3, and IGFBP-4 and decreased serum IGFBP-1 and IGFBP-2 in post-menopausal women.