Maturation-dependent response of the piglet brain to scaled cortical impact
Maturation-dependent response of the piglet brain to scaled cortical impact
复制标题
DOI：
10.3171/jns.2000.93.3.0455
复制
发表时间：
2000-09-01
影响因子：
4.1
通讯作者：
Raghupathi, R
中科院分区：
文献类型：
--
作者：
Duhaime, AC;Margulies, SS;Raghupathi, R
对象。本研究的目的是研究局灶性脑损伤脑回模型的成熟阶段与大脑对机械损伤的反应之间的关系。损伤反应的年龄依赖性差异可能解释婴儿和幼儿中出现的某些独特临床综合征，并将决定特定疗法在不同年龄是否特别有效，甚至适得其反。为了给不同年龄的动物提供比例相同的损伤输入，作者开发了一种局灶性挫伤仔猪模型，通过使用特定体积的快速皮层位移，精确地按比例调整大脑的大小和尺寸变化。使用该模型，比较了创伤时5日龄、1个月龄和4个月龄仔猪在损伤后7天对尺度局灶性皮质冲击的组织学反应。尽管有类似的损伤输入和稳定的生理参数，不同年龄的动物半球损伤的百分比差异显着，最小的动物维持最小的损伤（分别为0.8%，8.4%和21.5%，分别为5天，1个月和4个月的动物，p = 0.0018）。这些结果表明，对于这种特殊的局灶性损伤类型和严重程度，机械损伤的易感性在成熟过程中逐渐增加。由于其发育和形态与人脑相似，仔猪脑在模拟机械损伤的年龄特异性反应方面具有明显的优势。导致细胞死亡或修复的途径的差异可能与设计适合不同年龄患者的治疗方法有关。
Object. The goal of this study was to investigate the relationship between maturational stage and the brain's response to mechanical trauma in a gyrencephalic model of focal brain injury. Age-dependent differences in injury response might explain certain unique clinical syndromes seen in infants and young children and would determine whether specific therapies might be particularly effective or even counterproductive at different ages.Methods. To deliver proportionally identical injury inputs to animals of different ages, the authors have developed a piglet model of focal contusion injury by using specific volumes of rapid cortical displacement that are precisely scaled to changes in size and dimensions of the growing brain. Using this model, the histological response to a scaled focal cortical impact was compared at 7 days after injury in piglets that were 5 days, 1 month, and 4 months of age at the time of trauma. Despite comparable injury inputs and stable physiological parameters, the percentage of hemisphere injured differed significantly among ages, with the youngest animals sustaining the smallest lesions (0.8%, 8.4%, and 21.5%, for 5-day-, 1-month-, and 4-month-old animals, respectively, p = 0.0018).Conclusions. These results demonstrate that, for this particular focal injury type and severity, vulnerability to mechanical trauma increases progressively during maturation. Because of its developmental and morphological similarity to the human brain, the piglet brain provides distinct advantages in modeling age-specific responses to mechanical trauma. Differences in pathways leading to cell death or repair may be relevant to designing therapies appropriate for patients of different ages.