Inducement of apoptosis by cucurbitacin E, a tetracyclic triterpenes, through death receptor 5 in human cervical cancer cell lines.
Inducement of apoptosis by cucurbitacin E, a tetracyclic triterpenes, through death receptor 5 in human cervical cancer cell lines.
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DOI：
10.1038/cddiscovery.2017.14
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发表时间：
2017
影响因子：
7
通讯作者：
Hsu YC
中科院分区：
文献类型：
--
作者：
Cheng YM;Shen CJ;Chang CC;Chou CY;Tsai CC;Hsu YC
宫颈癌是女性中最常见的恶性肿瘤，根治或子宫切除术是其主要治疗方法。curcubit素E （cucubit素E）是从瓜地中提取的一种天然化合物类药物。以前被证明是一种抗肿瘤和有效的化学预防剂对几种类型的肿瘤。本研究探讨了Cu E对宫颈癌细胞株（HeLa和Ca Ski）的抗增殖和凋亡作用。结果表明，细胞毒性与细胞凋亡积累有关，而与坏死无关。Cu E可诱导细胞凋亡，上调死亡受体5 （DR5）的表达。此外，DR5基因在细胞凋亡中的激活作用均随Cu E剂量的增加而成比例增加；然而，有丝分裂的延迟也依赖于Cu E在癌细胞中的治疗量。这些结果表明，铜E可能通过上调DR5基因的表达，通过细胞凋亡延缓癌细胞的生长。
Cervical cancer is the most common malignancy in women, for which conization or hysterectomy are the main therapy. Curcubitacin E (Cu E) is a natural compound-based drug which from the Guadi (climbing stem of Cucumic melo L). Previously shown to be an anti-tumor as well as a potent chemopreventive agent against several types of tumors. The present study, investigated anti-proliferation and apoptosis induced by Cu E in cervical cancer cell lines (HeLa and Ca Ski). The results indicate that the cytotoxicity is associated with accumulation in apoptosis but not necrosis. Cu E produced apoptosis as well as the up-regulation the expression of death receptor 5 (DR5). In addition, the DR5 gene activation in apoptosis, both effects increased proportionally with the dose of Cu E; however, mitosis delay was also dependant on the amount of Cu E treatment in the cancer cells. These results indicate that Cu E may delay cancer cell growth by apoptosis via upregulation of DR5 gene expression.