Analysis of segregation distortion and its relationship to hybrid barriers in rice.
Analysis of segregation distortion and its relationship to hybrid barriers in rice.
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DOI：
10.1186/s12284-014-0003-8
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Koh HJ
中科院分区：
文献类型：
--
作者：
Reflinur;Kim B;Jang SM;Chu SH;Bordiya Y;Akter MB;Lee J;Chin JH;Koh HJ
分离扭曲(SD)是由不同基因型杂交产生的作图群体中经常观察到的现象。在本研究中，利用来自大三白(水稻)和伊洛姆白(水稻)的正反交F2和BC1F1作图群体构建了10个遗传连锁图谱，以确定这两个亚种间遗传致病机制的遗传因素的分布、效应和程度。本研究中检测到的SD基因座受男性功能、女性功能和合子选择的影响。最显著的SD基因座位于第3染色体(通过雄配子传递)、第5染色体(通过雄配子传递)和第6染色体(通过雌配子传递)。经卡方检验，BC1F_1代群体SD水平低于F_2代群体。Dasanbyeo等位基因在F2和BC1F1群体中都有较高的传递频率，这表明在水稻亚种间杂交中，Indica等位基因具有强烈的优势。本研究中的SD基因座与先前报道的生殖障碍基因座相对应。此外，在第2和12号染色体上还检测到了新的SD基因座。识别SD在遗传图谱群体中的分布和引起SD的遗传因素的影响，为在全基因组中寻找新的SD基因座及其与生殖障碍的关系提供了机会。这为进一步阐明水稻SD的遗传机制奠定了基础，并将在分子育种中发挥重要作用。本文的在线版本(doi：10.1186/s12284-0140003-8)包含补充材料，授权用户可以使用。
Segregation distortion (SD) is a frequently observed occurrence in mapping populations generated from crosses involving divergent genotypes. In the present study, ten genetic linkage maps constructed from reciprocal F2 and BC1F1 mapping populations derived from the parents Dasanbyeo (indica) and Ilpumbyeo (japonica) were used to identify the distribution, effect, and magnitude of the genetic factors underlying the mechanisms of SD between the two subspecies. SD loci detected in the present study were affected by male function, female function, and zygotic selection. The most pronounced SD loci were mapped to chromosome 3 (transmitted through male gametes), chromosome 5 (transmitted through male gametes), and chromosome 6 (transmitted through female gametes). The level of SD in BC1F1 populations which defined by chi-square value independence multiple tests was relatively low in comparison to F2 populations. Dasanbyeo alleles were transmitted at a higher frequency in both F2 and BC1F1 populations, suggesting that indic a alleles are strongly favored in inter-subspecific crosses in rice. SD loci in the present study corresponded to previously reported loci for reproductive barriers. In addition, new SD loci were detected on chromosomes 2 and 12. The identification of the distribution of SD and the effect of genetic factors causing SD in genetic mapping populations provides an opportunity to survey the whole genome for new SD loci and their relationships to reproductive barriers. This provides a basis for future research on the elucidation of the genetic mechanisms underlying SD in rice, and will be useful in molecular breeding programs. The online version of this article (doi:10.1186/s12284-014-0003-8) contains supplementary material, which is available to authorized users.