ROS scavenging capacity and neuroprotective effect of α-mangostin against 3-nitropropionic acid in cerebellar granule neurons
ROS scavenging capacity and neuroprotective effect of α-mangostin against 3-nitropropionic acid in cerebellar granule neurons
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DOI：
10.1016/j.etp.2008.11.002
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发表时间：
2009-09-01
影响因子：
--
通讯作者：
Mata, Rachel
中科院分区：
文献类型：
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作者：
Pedraza-Chaverri, Jose;Maria Reyes-Fermin, Laura;Mata, Rachel
α-Mangostin是一种从山竹果中分离出来的具有抗氧化特性的氧杂蒽酮。研究了原代培养的小脑颗粒神经元（CGNs）对活性氧（ROS）的清除能力以及α-倒捻子素（alpha-mangostin）对线粒体毒素3-硝基丙酸（3-NP）的保护作用。结果表明，α-倒捻子素对单线态氧、超氧阴离子和过氧亚硝酸根阴离子具有浓度依赖性的清除作用。相比之下，α-倒捻子素不能清除羟基自由基和过氧化氢。此外，α-倒捻子素能够以浓度依赖性方式改善3-NP诱导的神经元死亡。这种保护作用与3-NP诱导的活性氧形成的改善有关。可以得出结论，α-倒捻子素能够直接抑制几种ROS，并且在CGNs的原代培养物中对3-NP具有神经保护作用，这与其改善3-NP诱导的ROS产生的能力有关。(C)2008年Elsevier GmbH。All rights reserved.
alpha-Mangostin is a xanthone with antioxidant properties isolated from mangosteen fruit. The reactive oxygen species (ROS) scavenging capacity and the potential protective effect of alpha-mangostin against the mitochondrial toxin 3-nitropropionic acid (3-NP) in primary cultures of cerebellar granule neurons (CGNs) were studied in the present work. It was found that alpha-mangostin was able to scavenge in a concentration-dependent way singlet oxygen, superoxide anion and peroxynitrite anion. In contrast, alpha-mangostin was unable to scavenge hydroxyl radicals and hydrogen peroxide. Furthermore, alpha-mangostin was able to ameliorate in a concentration-dependent way the neuronal death induced by 3-NP. This protective effect was associated with an amelioration of 3-NP-induced reactive oxygen species formation. It is concluded that alpha-mangostin is able to scavenge directly several ROS and has a neuroprotective effect against 3-NP in primary cultures of CGNs, which is associated with its ability to ameliorate 3-NP-induced ROS production. (C) 2008 Elsevier GmbH. All rights reserved.