THE ROLE OF 2',3'-CYCLIC PHOSPHODIESTERS IN THE BOVINE PANCREATIC RIBONUCLEASE-A CATALYZED CLEAVAGE OF RNA - INTERMEDIATES OR PRODUCTS
THE ROLE OF 2',3'-CYCLIC PHOSPHODIESTERS IN THE BOVINE PANCREATIC RIBONUCLEASE-A CATALYZED CLEAVAGE OF RNA - INTERMEDIATES OR PRODUCTS
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DOI：
10.1016/0014-5793(93)80654-d
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发表时间：
1993-11-01
期刊：
影响因子：
3.5
通讯作者：
NOGUES, MV
中科院分区：
文献类型：
--
作者：
CUCHILLO, CM;PARES, X;NOGUES, MV
There is a considerable degree of ambiguity in the literature regarding the role of the 2',3'-cyclic phosphodiesters formed during the reaction of RNA cleavage catalysed by ribonuclease. Usually the reaction is considered to take place in two steps: in the first step there is a transphosphorylation of the RNA 3',5'-phosphodiester bond broken yielding a 2',3-cyclic phosphodiester which in the second step is hydrolysed to a 3'-nucleotide. Although in many occasions, either explicitly or implicitly, the reaction is treated as taking place sequentially, this is not the case as it has been shown that the 2',3'-phosphodiesters are actually released to the medium as true products of the reaction and that no hydrolysis of these cyclic compounds takes place until all the susceptible 3',5'-phosphodiester bonds have been cyclised. Comparison of the hydrolysis and alcoholysis of the 2,3'-phosphodiesters catalysed by RNase A indicates that the hydrolysis reaction has to be considered formally as a special case of the transphosphorylation back reaction in which the R group of the R-OH substrate is just H. It is thus concluded that the 2',3'-cyclic phosphodiesters formed in the ribonuclease A reaction are true products of the transphosphorylation reaction and not intermediates as usually considered.
