Fos and Jun expression in rat supraoptic nucleus neurons after acute vs. repeated osmotic stimulation.
Fos and Jun expression in rat supraoptic nucleus neurons after acute vs. repeated osmotic stimulation.
复制标题
急性与重复渗透刺激后大鼠视上核神经元中 Fos 和 Jun 的表达。
DOI：
10.1016/s0006-8993(96)01216-4
复制
发表时间：
1997
期刊：
影响因子：
2.9
通讯作者：
McCabe,JT
中科院分区：
文献类型：
--
作者：
Wang,K;Guldenaar,SE;McCabe,JT
应用免疫荧光双标技术，分析了高渗盐水注射后视上核(SON)神经元内Fos和Jun出现的时程变化。Fos、Jun免疫染色在30min内出现，90~120min达高峰，4~5h后消失。在各时间点(注射后30、60、120、180、240min)观察Fos和Jun免疫共染色(标记神经元90%共定位染色)。在注射生理盐水后4h，部分大鼠再次注射生理盐水或高渗盐水。再次注射生理盐水90min后，SON神经元无Fos/Jun免疫染色，而高渗盐水诱导的SON神经元Fos/Jun联合染色仅占17%。在剩余的SON细胞中，23%的细胞仅对Fos染色，4%的细胞仅对Jun染色。尽管提供了有效的第二渗透刺激(由血浆渗透压和钠含量确定)，SON神经元的Fos/Jun免疫染色较少，Jun表达显著减少，Fos免疫染色明显减弱。这些结果是在已知的负反馈机制的背景下讨论的，该机制控制这些转录因子的重新表达。
Using a double-label immunofluorescence method, we analyzed the time course of the appearance of Fos and Jun in the nuclei of supraoptic nucleus (SON) neurons following intraperitoneal injection of hypertonic saline. Fos and Jun immunostaining appeared within 30 min, peaked at 90–120 min, and disappeared 4–5 h later. At all time points (30, 60, 120, 180, 240 min postinjection), colocalized Fos and Jun immunostaining was observed (>90% colocalized staining in labeled neurons). At 4 h post-saline injection, some rats received a second injection of normal or hypertonic saline. A second injection of normal saline resulted in no Fos/Jun immunostaining 90 min later, while hypertonic saline induced combined Fos/Jun staining in only 17% of SON neurons. Of the remaining SON cells, 23% had staining to Fos alone and 4% of the cells stained for Jun only. In spite of the delivery of an effective second osmotic stimulus, determined by assessment of plasma osmolality and sodium content, SON neurons exhibited less colocalized Fos/Jun immunostaining, dramatically less Jun expression, and substantial, but attenuated, immunostaining for Fos. These results are discussed in the context of known negative feedback mechanisms that control the re-expression of these transcription factors.