Trophoblasts and Decidual Stromal Cells Regulate Decidual NK Cell Functions Via Interaction between Collagen and LAIR-1
Trophoblasts and Decidual Stromal Cells Regulate Decidual NK Cell Functions Via Interaction between Collagen and LAIR-1
复制标题
滋养细胞和蜕膜基质细胞通过胶原蛋白和 LAIR-1 之间的相互作用调节蜕膜 NK 细胞功能。
DOI：
10.1111/aji.12211
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发表时间：
2014-04-01
影响因子：
3.6
通讯作者：
Li, Da-Jin
中科院分区：
文献类型：
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作者：
Fu, Qiang;Tao, Yu;Li, Da-Jin
为了确定母胎界面胶原蛋白对蜕膜自然杀伤细胞（dNK）功能的影响，研究方法从正常妊娠和流产中收集蜕膜和绒毛样本。分别用流式细胞术和酶联免疫吸附试验（ELISA）分析细胞表型和细胞因子的产生。结果正常妊娠组母胎界面胶原和LAIR-1的表达均高于流产组。与HTR-8/DSCs共培养的dNK上调LAIR-1对dNK的作用，而LAIR-1的竞争性抑制剂LAIR-2可减弱dNK的作用。胶原下调细胞表面受体活性和细胞内穿孔素的表达，而上调dNK上抑制性受体的表达。与HTR-8/DSC的dNK共培养降低dNK的穿孔素表达和Th1型细胞因子产生，这可以被LAIR-2废除。此外，通过shRNA沉默HTR-8/DSC中的胶原蛋白显着减弱了对dNK的调节。结论滋养细胞和DSC通过分泌胶原蛋白来调节蜕膜NK细胞的功能，这参与了人类妊娠的维持。
ProblemTo determine the effect of collagen from maternal-fetal interface on decidual natural killer cell (dNK) function.Method of studyDecidual and villous samples were collected from normal pregnancy and miscarriage. The phenotype and cytokine production were analyzed, respectively, by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Co-culture was established to investigate the effect of trophoblasts and decidual stromal cells (DSCs) on dNKs.ResultsMaternal-fetal interface of normal pregnancy showed higher collagen and LAIR-1 expression than that of miscarriage. Co-culture of dNKs with HTR-8/DSCs up-regulated LAIR-1 on dNKs that could be attenuated by pre-treatment with LAIR-2, a competitive inhibitor of LAIR-1. Collagen down-regulated expression of cell surface receptor activity and intracellular perforin, while it up-regulated expression of suppressive receptor on dNKs. Co-culture of dNKs with HTR-8/DSCs decreased perforin expression and Th1-type cytokines production by dNKs, which could be abrogated by LAIR-2. In addition, silence of collagen in HTR-8/DSCs by shRNA significantly attenuated regulation on dNKs.ConclusionTrophoblasts and DSCs regulate decidual NK cell functions via secreting collagen, which is involved in the maintenance of human pregnancy.