Blebbistatin inhibits sphingosylphosphorylcholine-induced contraction of collagen-gel fiber populated by vascular smooth-muscle cells.
Blebbistatin inhibits sphingosylphosphorylcholine-induced contraction of collagen-gel fiber populated by vascular smooth-muscle cells.
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DOI：
10.1254/jphs.sc0060104
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发表时间：
2006
影响因子：
3.5
通讯作者：
Takeshi Katayama;S. Yoshiyama;Hideyuki Tanaka;Hong Hui Wang;A. Nakamura;K. Kohama
中科院分区：
文献类型：
--
作者：
Takeshi Katayama;S. Yoshiyama;Hideyuki Tanaka;Hong Hui Wang;A. Nakamura;K. Kohama
我们制备了一种细胞填充的胶原凝胶纤维，包括来自豚鼠基底动脉的GbaSM-4细胞。在电子显微镜观察中，这种纤维往往是一种分化的收缩表型。鞘氨醇磷酸胆碱（SPC）可诱导纤维收缩（EC 50 = 0.70 +/- 0.05 μ M），而blebbistatin可抑制SPC诱导的收缩（IC 50 = 22.8 +/- 1.26 μ M）。20 kD肌球蛋白轻链（MLC 20）的磷酸化在提供1 μ M SPC的GbaSM-4细胞中显著增加（P<0.05），其在1至100 μ M blebbistatin存在下得以维持。这些结果表明，即使MLC 20被磷酸化，血管平滑肌也可以松弛。
We prepared a cell-populated collagen-gel fiber including GbaSM-4 cells derived from the basilar artery of guinea pigs. This fiber tended to be a differentiated contractile phenotype in electron-microscope observations. Sphingosylphosphorylcholine (SPC) can induce contraction of the fiber (EC50 = 0.70 +/- 0.05 microM), and blebbistatin can inhibit the SPC-induced contraction (IC50 = 22.8 +/- 1.26 microM). Phosphorylation of the 20 kD myosin light chain (MLC20) significantly increased in GbaSM-4 cells provided with 1 microM SPC (P<0.05), which was maintained in the presence of 1 to 100 microM blebbistatin. These results suggest that vascular smooth muscle can relax even if MLC20 is phosphorylated.