Characterization of Mesenchymal Stem Cells From Human Vocal Fold Fibroblasts
Characterization of Mesenchymal Stem Cells From Human Vocal Fold Fibroblasts
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DOI：
10.1002/lary.20797
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发表时间：
2010-03-01
期刊：
影响因子：
2.6
通讯作者：
Thibeault, Susan L.
中科院分区：
文献类型：
--
作者：
Hanson, Summer E.;Kim, Jaehyup;Thibeault, Susan L.
目的/假设：间充质干细胞（MSC）最初分离自骨髓（BM），是成纤维细胞样细胞，现在被认为存在于许多组织的基质成分中。MSC的特征在于一定的标准集，包括它们的生长培养特征、细胞表面标志物的组合和沿沿着多种间充质组织谱系分化的能力。我们假设，从固有层分离的人声带成纤维细胞（hVFF）符合标准，建立了定义间充质干细胞和功能相似的骨髓和脂肪组织来源的间充质干细胞。研究设计：在体外study.Methods：hVFF以前来自人声带组织。MSC来源于脂肪组织（AT），和健康供体的BM，基于它们对培养皿的附着和它们的形态，并在培养物中扩增。分析细胞在BM衍生的MSC上鉴定的标准细胞表面标志物和分化成间充质谱系细胞的能力（即，脂肪、骨和软骨）。我们研究了这些细胞的免疫表型之前和之后的干扰素-γ（INF-γ）stimulation.Results：hVFF显示细胞表面标志物和多潜能分化能力的MSC的特征。此外，这些细胞表现出类似的人类白细胞抗原和共刺激分子的表达模式，刺激后与INF-γ相比，骨髓和AT来源的骨髓间充质干细胞。结论：根据我们的研究结果，来自固有层的hVFF具有相同的细胞表面标志物，免疫表型特征和分化潜力的BM和AT来源的骨髓间充质干细胞。我们认为声带成纤维细胞是存在于声带固有层的间充质干细胞。
Objectives/Hypothesis: Mesenchymal stem cells (MSCs) originally isolated from bone marrow (BM), are fibroblast-looking cells that are now assumed to be present in the stromal component of many tissues. MSCs are characterized by a certain set of criteria, including their growth culture characteristics, a combination of cell surface markers, and the ability to differentiate along multiple mesenchymal tissue lineages. We hypothesized that human vocal fold fibroblasts (hVFF) isolated from the lamina propria meet the criteria established to define MSCs and are functionally similar to MSCs derived from BM and adipose tissue.Study Design: In vitro study.Methods: hVFF were previously derived from human vocal fold tissues. MSCs were derived from adipose tissue (AT), and BM of healthy donors based on their attachment to culture dishes and their morphology and expanded in culture. Cells were analyzed for standard cell surface markers identified on BM-derived MSCs and the ability to differentiate into cells of mesenchymal lineage (i.e., fat, bone, and cartilage). We investigated the immunophenotype of these cells before and after interferon-gamma (INF-gamma) stimulation.Results: hVFF displayed cell surface markers and multipotent differentiation capacity characteristic of MSCs. Furthermore, these cells exhibited similar patterns of expression of human leukocyte antigen and costimulatory molecules, after stimulation with INF-gamma compared to MSCs derived from BM and AT.Conclusions: Based on our findings, hVFF derived from lamina propria have the same cell surface markers, immunophenotypic characteristics, and differentiation potential as BM- and AT-derived MSCs. We propose that vocal fold fibroblasts are MSCs resident in the vocal fold lamina propria.