Acetylcholinesterase biosensor based on chitosan/prussian blue/multiwall carbon nanotubes/hollow gold nanospheres nanocomposite film by one-step electrodeposition.
Acetylcholinesterase biosensor based on chitosan/prussian blue/multiwall carbon nanotubes/hollow gold nanospheres nanocomposite film by one-step electrodeposition.
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DOI：
10.1016/j.bios.2012.10.058
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发表时间：
2013-04
影响因子：
12.6
通讯作者：
Chen Zhai;Xia Sun;Wenping Zhao;Zhili Gong;Xiangyou Wang
中科院分区：
文献类型：
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作者：
Chen Zhai;Xia Sun;Wenping Zhao;Zhili Gong;Xiangyou Wang
采用一步电沉积法在金电极表面制备壳聚糖-普鲁士蓝-多壁碳纳米管-中空金纳米球（Chit-PB-MWNTs-HGNs）膜，并将乙酰胆碱酯酶（AChE）和Nafion修饰在该膜上，制备了AChE生物传感器。将MWNTs和HGNs组装到Chit-PB杂化膜中，促进了电子转移反应，增强了电化学响应，改善了电极表面的微结构。采用扫描电镜、透射电镜、循环伏安法和电化学阻抗谱等研究了复合材料的形貌和电化学性能。优化了pH值、酶负载量和抑制时间等影响生物传感器响应的参数。基于农药对乙酰胆碱酯酶活性的抑制作用，以马拉硫磷、毒死蜱、久效磷和克百威为模型化合物，该生物传感器具有检测范围宽、检出限低、重现性好、稳定性高等特点。此外，AChE/Chit-PB-MWNTs-HGNs/Au生物传感器还可用于实际样品的直接分析，有望成为农药分析的新工具。
In this paper, chitosan–prussian blue–multiwall carbon nanotubes-hollow gold nanospheres (Chit–PB–MWNTs–HGNs) film was fabricated onto the gold electrode surface by one-step electrodeposition method; and then acetylcholinesterase (AChE) and Nafion were modified onto the film to prepare an AChE biosensor. Incorporating MWNTs and HGNs into Chit–PB hybrid film promoted electron transfer reaction, enhanced the electrochemical response and improved the microarchitecture of the electrode surface. The morphologies and electrochemistry properties of the composite were investigated by using scanning electron microscopy, transmission electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy, respectively. Parameters affecting the biosensor response such as pH, enzyme loading and inhibition time were optimized. Based on the inhibition of pesticides on the AChE activity, using malathion, chlorpyrifos, monocrotophos and carbofuran as model compounds, this biosensor showed a wide range, low detection limit, good reproducibility and high stability. Moreover, AChE/Chit–PB–MWNTs–HGNs/Au biosensor can also be used for direct analysis of practical samples, which would be a new promising tool for pesticide analysis.