Comparative analysis of regulatory roles of P38 signaling pathway in 8 types liver cell during liver regeneration
Comparative analysis of regulatory roles of P38 signaling pathway in 8 types liver cell during liver regeneration
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8种肝细胞P38信号通路在肝再生过程中调控作用的比较分析
DOI：
10.1016/j.gene.2016.08.056
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发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
Xu Cunshuan
中科院分区：
文献类型：
--
作者：
Yang Xianguang;Zhu Lin;Zhao Weiming;Shi Yaohuang;He Chuncui;Xu Cunshuan
P38 MAPK信号通路与细胞增殖、凋亡、分化、存活、死亡等密切相关，但P38 MAPK信号通路在肝再生（LR）中的调控机制尚不清楚。为了进一步揭示P38 MAPK信号通路在大鼠肝再生中的作用，利用IPA软件及相关数据库构建P38 MAPK信号通路，并通过相关文献资料进行验证。应用Rat Genome 230 2.0 Array进一步分析P38 MAPK信号通路相关基因在8种细胞中的表达变化，结果显示P38 MAPK信号通路中有95个基因发生了显著变化。H-聚类分析显示肝细胞（HC）、小凹细胞（PC）、卵圆细胞（OC）和胆管上皮细胞（BEC）聚在一起，枯否细胞（KC）、肝窦内皮细胞（SEC）、树突状细胞（DC）和肝星状细胞（HSC）聚在一起。应用IPA软件和表达分析系统探测器（EASE）进行功能富集分析，结果表明P38 MAPK信号通路主要参与LR过程中的细胞凋亡、细胞死亡、细胞增殖、细胞存活、细胞活力、活化、细胞周期进程、坏死、DNA合成等生理活动。综上所述，P38 MAPK信号通路通过多种信号通路调控LR的多种生理活性。
P38MAPK signaling pathway was closely related to cell proliferation, apoptosis, cell differentiation, cell survival, cell death, and so on. However, the regulatory mechanism of P38MAPK signaling pathway in liver regeneration (LR) was unclear. In order to further reveal the roles of P38MAPK signaling pathway in rat liver regeneration, Ingenuity Pathway Analysis (IPA) software and related data sites were used to construct P38MAPK signaling pathway, and the pathway was confirmed by relevant documents literature. The expression changes of P38MAPK signaling pathway-related gene in eight type cells were further analyzed by Rat Genome 230 2.0 Array, and the results showed that 95 genes in P38MAPK signaling pathway had significant changes. H-cluster analysis showed that hepatocyte cell (HC), pit cell (PC), oval cell (OC) and biliary epithelial cell (BEC) are clustered together; and the same as Kupffer cell (KC), sinusoidal endothelial cell (SEC), dendritic cell (DC) and hepatic stellate cell (HSC). IPA software and expression analysis systematic explorer (EASE) were applied to functional enrichment analysis, and the results showed that P38MAPK signaling pathway was mainly involved in apoptosis, cell death, cell proliferation, cell survival, cell viability, activation, cell cycle progression, necrosis, synthesis of DNA and other physical activity during LR. In conclusion, P38MAPK signaling pathway regulated various physiological activities of LR through multiple signaling pathways.